XB-CLONE-3548551
Clone Name: IMAGE:7716255
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976565 [+]
tcgaattcggccgagggttggagtagatcagttcctggttaagactggaacaatcactacctttgaacattcacataacatgcgtgttatgaagtttagt
gtcagccctgttgtgcgtgttgctgtggaagcaaagaatcctgctgatctccctaagttggttgagggtctgaaacgtctagctaaatcagaccccatgg
ttcagtgcatcattgaagaatctggcgagcatatcattgctggagctggggagctgcatttggagatttgcctgaaggatcttgaggaggatcatgcctg
cattccaataaagaaatctgatccagtcgtatcttaccgtgagactgtgagcgaggagtccaaccagatgtgcctgtcaaagtctcctaacaagcacaac
cgtctgtttatgaagtgtcgaccttttcctgatggtcttgctgaagacattgataaaggtgatgtatctgctcgccaggagttgaagactcgtgcccgct
acttggctgaaaagtacgaatgggatgtgactgaagctaggaagatttggtgttttggtcctgatggctctgggcccaacatacttactgatgttactaa
gggagttcagtatctgaatgaaatcaaagacagcgttgttgcaggattccagtgggctacaaaggagggtgtcctgtgtgaagaaaatttgagaggtgtt
cgttttgatgtccatgatgtaacacttcatgctgatgccatccatagaggaggaggacagatcattccaactgcacgccgcgtcttgtatgcttgtgtgt
tgactgctcagc
3' EST: CX976564 [+]cattaaatttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacat
ttacttccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcaggg
ttccaacagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtc
aaggaagttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatct
ccaggtagaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacag
gcaggtatgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctac
cacctgctcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttgga
atgatctgtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcaaattttcttcacacaggacaccct
cctttgtagcccactggaatcctgcaacaacgctgtctttgattttcatcagatactgaactccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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