XB-CLONE-3548543
Clone Name: IMAGE:7716017
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Gene:
golgb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976570 [+]
caaaacagggctaaaactatgatactgtgtactgcaccttcagtttaatatcacttcaaaatgcctggaatggtttagtctttggattggcaccaggaca
tctgttcagaaaagatcgcgaaggacatcccatagtattgaaattgcaatgtatacagacatgtacagaatatattgtttctgctaaacaacattcacta
ttaagtgtgatgttctgatgtacccttcgatctctgaagatgtaacctctctgacctcgtactgtcctaaaacacctttgttgccttttctataacccaa
agtctgatgttaaacaatagttgtgatttctgacaacatatgtaatctctagaatgccctgtgcccatttaattcggtatctatgtgcaatatggcagta
cttctgccagagagatccaagtatgtgtgtaagtagcacttctgttatgttagttatgtctatataatagtatgaggaccagaaaatccattaaaagtaa
tttattggaacctagatctgttaaaggaatatatacttctccattaagtattcctgcttgtttacaaatgaaaagcagatatatttgacgtttgcccatg
ccattattcctgtatacatttcatccataaatcatctaaataagcaggtcctataggcttgcagcatacataaactatgaatttatgaccatgtctcaat
acataatttctcagtgtgggagagtgccatgagacttttggaaaggctatgttaagtgttgatctttccttgctcattagggtaatnaaggctataatga
atagtgttataaagaatttatgttaaccaaatatacatgacctatttgaacagccttttgcatgttgaatgaaccagatctgttttt
3' EST: CX976569 [+]ttgggctgtaattgtactgtgtcaatcagtgaaaagtagaaacccatgttcccaattctaggacataagagatacattaagtcataaaaacacagaactg
agatcacatacaaaagccccagaggtcagtgcaatgaccagcctaacacacagaatgggtattacataaatatttcacctgcaaaccaaagaatgatcag
ttgcaataacagtaaatacaaatgaaatacactgcacatgaacacccatcatagatataaacataccagtttttctaaatcagatactgaaagaaatata
tagtgcaaagaaaaacaaaaaaagttcagacaacagaaggtaaagggctgataagagtaaaatgaggaaattaatgaggggactctgcaagctccccatg
gcactttaataccaatcatcttttcagaaatacaggaaaccccagataagtgttgttgagtaagaacaattagtattgctgggtagtgtaatgaataaca
atataggttttattttttaatgttagcaagtgacagggggcacaaagatcacataatggaagacaggtggttgctgtcattgtcagcaaagtaatcagat
ctttacacatggcagatatctgtataacaagaacaattttttgccacatctggcactgaggaacaacgatgatgactgtgtgcagaagagactggctgaa
cactgggtggtaaatagggaaagttgctgacctgtttgtttccttttgcatagagtcgtagattaatatattggcaccatgacatgatattgatttcttg
tttctgttctatttgatttaaaaaaagatttgagtgtgttttatatgtctttcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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