XB-CLONE-3548520
Clone Name: IMAGE:7716067
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Gene:
lpl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976585 [+]
caaagatcaaaaaaaaacataataaaatatctaattatatatgaagggttttgaaaataactaaatatatatatatacaagagagtaaaaaatgttttca
tagttctacaatatatacaaatacatcttacactaaactacatataaacagtaaataaataataacaagttgtttttctttattccgtaacagtcaaacc
attttattcatttgatatataatcagtataattattttatctcaaagcacagcgccaaattttcaactaaagaggcatattccagtttttgaaatgacag
atacacagaacacaagtgttttggcggtgatacagaatctaatcacaactcttaatgattccttacacaatgcaaaatcaggaagttgcttttcatgctc
ttattgttgcccctcatattcacatgcctacagtgacgctgatcatactaacaaaagcttgtggtgctacttgtgcttgatttacacaatttgagctgaa
tgctgtgtagtaggcatctgctattatgctagagttttaaaaagaattgtgggttgcattgtgtgtaaaaacatgacaatttgcagtcttttccagcttt
acattgcattgtatttgcatttgtaattgtgccttttaatttgaatttaacactctcttatgaacaagagtaccggtatgtggacttaaaagagctagta
ttttgttataacctcctctgtagtcattcccatcattaatgtgcctacagataaaacattctatactacaaaattctttttnatgttatttgattataga
catacaattatagaaagatcacttgtaggattatttaatctactttacaaaaaatcaacacagataatatatcatgctcta
3' EST: CX976584 [+]gaaaaatcagtaatactttccctagactcaatttcagaccagttcatagacatacaagggtaatttttttcgccatttcgccaatttttttgcagttttg
caaatttgaagtgaaacaggacagattcacacatcactacagttgatctatccaactaagaggacaattgtatgcctgttataggccaattccaaaatgc
tatagctgtctggtccaatctccccctcacattgtgggggagggagaacctgttcgaaatggtatacctgcccatatttttataggtttcaaaggcaata
tttaaaaaaaaaaaactacaaaaaaatgggcagccggccagcggcttacggtcctagatgccaattgacccccatttaaaagctggaaagaggtagaaga
ggaagtcaagtaaatcaaaaattataaagaataaataatgaaggccaattgcaaaattgatagaaatagggaatgctataacataactggatgataaagg
tgaaccactcctttaatgagatattagatatttatggagttaaggtttatttatattgtgatttttttcccatgattttgcacatatttagtgaaaatct
caaatagtttatttctatcatgttaagccaaatagtggcctaaatagactcaagtcacatatctcctttcattttaagttaataaaatgatgaatgaggt
aaaaaactggattatcaacttgcttgaatggtaacaatatatttcccattgaattctatggcatctcaacaggtttttatctttgattcaatttttaact
aaaatgctgaattctgactatggaaatacaacaggcatcataagccttgtaaaattagaacaaaaaacaccctgaaacatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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