XB-CLONE-3548517
Clone Name: IMAGE:7716115
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Gene:
adam28.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976587 [+]
taaagttgtacaaaacagtaaaagaagcattttccataaaagggcaaatatattgaaaaaatgtcatttccacagtagttggcactagatgtaccggcga
tcaaacaggtagggttaaaagccaatcggactgtaagggactaaccacgcggcacacaccaataaatagctcactcacgccccacaatacccggcactca
ctcattctcacaaatccctacactatcctttgacataaactatttacacaagtatttacagtacagaaagtgaaaaaaaaattatctttctaaaaccata
aaaaagagacaatgagaaatggcagatacccataatgccctgagaattcctttttgcacaaaagagataagtggacctgtaatgacccacattgtgctgc
ggtaccaggagcaacactgatatagtgtagtatagtatgactatgtatagtgtgactatgtacacagcgtgacaatcttctcccaaggcacagttgggat
tctcacatccatggtctgctactgccctccagtggaaaacagtggtggttaaagaccgataaaatggccctgtcattactgttaatattttagggggaac
tcaggcttccgaaccaaaatttgttatagagccccacacaaaacagaaacccctgatataactatcactgtaatctgtttcttcaaaaagtataattacc
atttttatctgctgaaatccaactgcaaaacagttcttctttttctgcatcactggaaatcctggcaggngaggaggaactaaaacattgatgttacaaa
ttgtaacaacttccccacagcttacagacagcatgtaggaactacataacccacaatgcattgcact
3' EST: CX976586 [+]atagggcgagaggacggggggagtaacctacagtcctctggatacaccatgctgcttcccctgctgctcctcactgccgggctcctgctcccccagcctg
cagcctctcaaaagttaactattgacccatattatgaagttgtattcccaaagaagattcatgcccagcacaagagagatgttcagggcatgtatcctga
agtggtgcaatatggcatacacatgggagggaagcatctgatcttacacctagagaagaatgaagacctgctgtctgcaaactacactgagacttactac
ctgtcagatgggacaccagtaacaaccagccctaaaattcagaatcactgctactatcaagggaatgtgaagaatgataatgggtccctggcgagcatta
gtacatgcagtgggctaagaggcctcatccagactgaaggccaaatgtatctaattgagcctctgaaagacacagacagcgagtcccatgctgtgtatga
ggcccagatggagacacccaaaacttgtggtgtggacaatacaacatatagagaggaaattgttgtcaagaaatctcgttccggaagctctgctgagcaa
cagcaaattctgaaggcccagaagtacatccgcttggttgttgttgcagataattcaatgtttattaagtacaacaagagtatcaccaacgtgaagataa
gaatcttcgaggttataaattttgtcaatgtggtgtataaggccataaatacctttgttgctctgatcgggattgaggtttggaacacgacgggacagtt
ttaagtggtgacctcggcctctgncgatctggctgccttcgctgactggagaaagatgttcttctgcgtaggacg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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