XB-CLONE-3548505
Clone Name: IMAGE:7716307
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Gene:
akr7a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976595 [+]
cggtcccgtccgtgccctcatgtctctctctgcagccgccgccccccagctgcccggcaccgtactgggcaccatggagttcgggaggcggatggacgag
aagtgcagcggtgagacggtgcgggactttctcaggccggccatgaggagttggacacggcgctaatgtacgcggagggagagacggagcggatactggg
gcgaatggaactcggctcccaagtcaaaatggcaaccaaagcaaatccctggggagggaataccctgaagcccgagagtgtgcgccaacaactggagaag
tctcttcagcaactgcaaactcccagcgtccatctcttctacctgcacgcgcccgatcaccaaactcccgtagaggagaccctgggcgcttgccaacagc
tgtaccaggagggcaagttcaaggaactggggctgtccaattatgctgcctgggaggtgatggaaatttactgcatttgcaaacagaggaattgggtgct
gcccactgtgtaccagggcatgtacaatgcaaccacaaggcaagtggagacagagcttctgccctgcctgaggcaactgggaatgaggttctatgcctac
aacccactggctggggggctgctgaccgggaggtacaagtacgaggataaagagaaggaacaggcctcgtgcagattttttggaaacagctgggcagaag
tctacaggaacagatactggaagaaacatcactttgatgccattgatctggtgcagaactccctgcaggaggcatacgggtcagagaggcctagcctgac
gtctgccgctc
3' EST: CX976594 [+]gaggctgcacctcccacccaggtccaactcccagtgcacccagttcccacttccggtgcaaaaataaagcatttagtgcaaatccctaggaatcaactga
aatcagcagctggccaatcagatcaggccgccgttaccctagtgataaggctgtgattagcggaaataattaggacattcgtgggcgaccaggttccagg
cttcgttgaaagctgccacaactggtgggagtaggggccccccttccagccccgccatgttctgcttgtactgctccgtactggacattcccaggatcac
tgcatcgccgaggctgccctgcagtttggagtggtggtacatccatcggagagcggcagacgtcaggctaggcctctctgacccgtatgcctcctgcagg
gagttctgcaccagatcaatggcatcaaagtgatgtttcttccagtatctgttcctgtagacttctgcccagctgtttccaaaaaatctgcacgaggcct
gttccttctctttatcctcgtacttgtacctcccggtcagcagccccccagccagtgggttgtaggcatagaacctcattcccagttgcctcaggcaggg
cagaagctctgtctccacttgccttgtggttgcattgtacatgccctggtacacagtgggcagcacccaattcctctgtttgcaaatgcagtaaatttcc
atcacctcccaggcagcataattggacagccccagttccttgaacttgccctcctggtacagctgttggcaagcgcccagggtctcctctacgggagttt
ggtgatcgggcgcgtgcaggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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