XB-CLONE-3548469
Clone Name: IMAGE:7716359
|
Gene:
faxdc2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX976624 [+]
tttaggcaaaattagaacataattgtggcacacgagttagttgtgattagtgtaagaatatgatgctgttgcacccacccaatcccactcgtcactactg
aataatgtacatacggtaacatccgtacctaatgggccactatggtgcacagtgatgtcagtgttctcaatggaccctaaatctacagctaaatatccac
agacactgggttttttttctatgcacctacagccatgaacaatttctaaccttctaattatcatttaatcacagagataattagtatcataagtcagtca
aaaactggagtgggtgccccaacacacccaaacccgctggatgttgctagtacattagcacaacattaagggcaaaccatatccttgcaggtctctgttc
aagagggacaacttctcttctaacatttccccctttagactattgggactttttaggggtgtcaggaatgctctgacttagtggtgtaaggttcattaat
agaatgtgtctttcataagccttggtttgcttgaacatcagatctgttccatgcagatgatccaataccccaagtactccgtagcactggttaaacttga
gatggtgaaaatcatggaactctggagaaggcaggaaaggcaggtggtagccacagtgtgagatggtggtggtgatcaaagccaggcagaaccacagcat
ggttgtggccacgtgggatcccattaccatagggccgaccatggatggcagcatgttggaaaaaatatgctccaggggatgtgcatacaggcacaccact
cctac
3' EST: CX976623 [+]gcgagaggcgtcggagtcaggatggccaggtgtgggactctgtaaagaagacagcttttgtcctgggcactgggcttctcatctttgctgcctttaggaa
ttccgtgacatggcacttgcagcagttttggggagcctcgggggacttttggcaagcacagtgggggaagctgcacactttctttgatggaaatcaactg
gcgttgttctgtttgggtaccatgctgatgcccacgctttccttctgggtcttcaatgccatgctgatgcttatcgacctcactggaaagccatacttca
tcaccaaataccggatccagcctgggaagaacgaccccgtggatccagccaagctccgacaggccgtgatcactgtgcttgccaatcaggtctttctctc
ctttcccatgatcgttctcatgtatcccatcatgctgtggcgaggaaatccctgtggccctgagctgcctacattccattgggtccttttggaactcaca
gtcttcgcgctggttgaggaaattctcttttattattcacaccggctggtccatcaccctctattgtacaagcgcatccacaagaagcaccatgaatgga
cggcacctgtaggagtggtgtgcctgtatgcacatcccctggagcatattttttccaacatgctgccatccatggtcggccctatggtaatgggatccca
cgtggccacaaccatgctgtggttctgcctggctttgatcaccaccaccatctcacactgtggctaccacctgcctttcctgccttctccagagttccat
gattttcaccatctcaagtttaaccagtgctacggagtacttggggtattggatcatctgcatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: