XB-CLONE-3548365
Clone Name: IMAGE:7716180
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976705 [+]
agagagactagtctctctccttttttagaccaaaatctctctctctctcgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaac
gccgccatgcaatcccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatc
tgtccatgtcttactatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaa
gttcctcaaataccaaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcag
gccgctctgcagctggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaat
ctgagtacttggaggaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaacggcatgggcgagta
cctgttcgacaagcacaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtctctttactat
ctgtgtggattacaccatagctcccatgcccctgttgacacctttttatcttgaggatgatgggaataaatctgtgctattactgttttgaatcatgcag
aagctgctgtgcttgtcaacatgttttcaataaagttttttcagca
3' EST: CX976704 [+]ttggaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcaaaacagtaatagcacagatttattcccatcatcctc
aagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttcc
accctgagagtgcgcttagctactctcccccagggtgtgcttgtcgaacaggtactcgcccatgccgttctgcggcaccccaaggcgcttcaggtttgtg
atgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagct
tgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttctt
gatatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttg
gccacatggtggagtgccacgtcatcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatgg
cggcttcgcagtcgctgttgaagttctgacgcacctgggattgcatggcggcgtttcggtgtttgctggtggcgcanaaaagtcacaaaaaaaagagtaa
tgcgagagagagagagattttggtctaaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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