XB-CLONE-3548340
Clone Name: IMAGE:7716326
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Gene:
slc13a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976723 [+]
ctgaaacctagggcacctagatatttcacccaatactgatagagccattaaaatgtttacattatttttcttaaataaatacaagtgagatacttgccct
gatacaaaaaattaagttcttagagagaactttttacactcttgcctgctgggtattgcccttattaacagtactattaactgataaggtccccaattaa
aagctttgcagagcagagtgagatgctaccttgactattctgtaggccggtagggtactcggcccatctgcttatagtcacagggtgggcacggcttgtg
cgtattcacggacactagagagtctgaactgtcaatctgtggattctggcagaagtcagtggggctgctgtaagatgccataaagaatcactgtttattg
ggctgatgggggactgtttgagtctcttggtagttgaaatgccagggtctattttgaattccagtctgaacctgtgccacatccaccactttatcatact
tctgaggctccttggcatccagttaaacacaactggccattaacagtccattacaaatgctaaggctgccctgtggagtttgcccaggatggaaatgtgc
ccaagtcgaacatgtaaaatccccagctgttaatggccagtgttatagtcagcacacccagaatgttgagcagtagtcctgcctttgccatgtcgataac
cttgagctgcccataagagaaggcaatggcattgggtggcgttgcaactggcagcataaaagccaaggaggccgatagtgtgcatggaagcatgatatac
agtggggtcagatggatt
3' EST: CX976722 [+]ttgcataaacgcattgccctaagagtgctgctgattgttggagttaaacctgccctcctgcttcttggattcatggtggtcacagccttcctttccatgt
ggatcagtaacacagccacaacggccatgatgatacctattgcacaggctgtgctgcagcagcttcacacctcggaagagagagtggacaagccgtcaga
agaaacaaaaaagaatgtgaatggaatggataatagcatgtatgaatcagtattgccaaatgggaaaatgatcctggcagtagaaaacacttatgctact
gtaaacgagggatttgagatgcaagatagtactaaagatcctgagccttccaagctagaaaaagaaagtattggcccaatagtgatagaacttgaggatg
aaaaacaaaaggaagagaagcagaaacttagattggagaagcacctgaagatctgcaaaggaatgacccttagtgtctgttattctgccagcattggtgg
aattgcaacattaactggcactaccccaaaccttgtaatgaaaggacaaatggacgagtaggctctttccagagaacaataatgtcatcaattttgcctc
ctggtttggttttgcatttcctacaatgtttgtacttctggcattatcctggttatggctccagttcatgtttttgggattcaactttaaaaagaatttt
gggtgcggtgggaattcagaacaaaaggaaaaggagaagagagcttttaaaatcatttctggngagcacaaaaagttgggcgccatgacatttgctgaaa
tatctgtgctggtgctatttatacttctggtgctgctgtggtttacaagggagcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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