XB-CLONE-3548219
Clone Name: IMAGE:7716194
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Gene:
aqp8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976804 [+]
ccgaggctcaaatgggtaacttgcccctggcctctcaaccaccaagaatcactcttagatgtgcagagaggaggtcgggaataagagaatgtggagtgag
aaggaagttggggagagaaaggtggtgagaggaaatttgagagtcagggaatgggagaggaaagggtggaaagcaatagattaaagagactgggatcttt
taacataagggccaagatggagtaggtggaaggggtgaataagatacaaaaggatattcataaggataataaagaaaagataggttaaagtgagtctatg
agggaattacaaggttctgttgattaaaggactaaggaatctcccagtgggccccactgtgcagtttgtaccatagaaagtggtgtattacagataccag
ctagtgcatttgtactaactgtgcatagagagggcccctgatagtcaggttctctggtgggcccagtccaacactgcctattattgtaaaagtacagata
gagaccgtatgagccagcatactaaagggtatttaaacccaattctaactaaggtgtattttgttgcctttgctgtatgtgcattcattaaaaactgtct
gtttgcaaatgtattctttaaatgtgtcaaatattcttaggaaattacgttcccctccctccagtccctgcaaaggagaataaagaaaagcctttaatat
cctataaatgatatccttataacttgcttattgatgttataagttatattaagtgcttaatgatgtcaaagcctttgtccttgtgctttaatatggtcat
aaacgtcatctgggacttataatagccttatattatacat
3' EST: CX976803 [+]acaaagacccccagaacattccatcctttccttatttctaatacaaattaaaatcagctctacaaaataattaccaaataaagaaataccagcagaagtc
aggaacaattaggccactgcctggtcctccctggcaccccccagttgcacccaccccgatggtgtatccagaggcccattagcaaaaacattgtccccag
gcctcattctcataatgggatcactgccagcttcttctcttgcctatatccctccatgtaggatggtgtggactgcactatttcttgtttggaaagagtt
aattctttgtacctatgggacctctcaaatattaggggttgtattgacttctttcagatttgtcagattactatcaggatcattcagccaatctaacatc
tgttggaagcagaaatgtacaatgtgcagaattccatgccagcctattataatggctgaggatgctgggaatttgtggcatggcaggagttacctgcctt
ttgctatacctgttgttgttgtatgagtgaatccggcatccccactgctattaagggcagtttttcaggggctttttccctgctataattattatgtatc
ttaattatagtacctttcaattatatagggaataatgtacccccttatgtataatataaggctattataagtcccagatgacgtttatgaccatattaaa
gcacaaggacaaaggctttgacatcattaagcacttaatataacttataacatcaataagcaagttataaggatatcatttataggatattaaaggcttt
tctttattctcctttgcagggactggagggaggggaacgtaatttcctaagaatatttgacacatttaaagaatacatttgcaaacagacagtttttaaa
tgatgcacatacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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