XB-CLONE-3548083
Clone Name: IMAGE:7716064
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Gene:
xbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976900 [+]
ccagtctgccccacggatcccctgcaccccttggacatggtggtcgtgggagcccccaaagtgatctttatccccgggaatcagtgtgagcaggttgacg
ccttgggatccgtaatgttacccttaagcagcccgagcagtccggagtcggtcagcaatgagctgccccccaggaagagacagagactcacccacctcac
cccggaggagaaagcgctcagaagaaaactaaaaaacagagtagcagcacagactgctcgggacaggaaaaaagccagaatgagtgaacttagcagcaag
tggtagatctagaaatggagaatgagaaactcttgcttgaaaaccaacttttaagagaaaaatctcacagcttgttgactgaaaaccaggagctacgcca
aagattaggccttagcaccctggaggtaaagaaggaagaagaattgcaggagctgaatcagtccagaaaagataaagtcaggccggagaccgggtccgct
gagtccgcagcactcagactatgtgcccctctgcagcaggagcaggcccagatgtctccgaacctgacagtgtctacatggatcctgacagccctgacac
ttcagactctgagtctgatctcttgttgggccttctggaaagcctggactcagacttgttgcttgcctatgaagaagcattcactggaagcccagatgaa
gagatcaacggagaagagtctgattccatatcctcctccccatcttctcctgtggggaccccatcagccaagctggaggccattaatgaactgatccgat
tcgatcacgtgtacaccaagccactaagcacaga
3' EST: CX976899 [+]aaatttcttaacctctaaaataaaaatggtggaaatatataatcatctcatttacacagtaaatttaaaataaaagtctgatctgcaggatactgtggtt
ctgtacattcctaccccctgctggtagaactttatatacaaggacatttgtggatcacaactaaaaatcttaacagaaaggcaaacttgctgagaatatg
tcattgcagatacaaacaaaatcttcttcttaaaatcagcagcagtgtcatgtaatctgacccgatctaaagacagtttaatgggaaagcatttaattcg
atcaaacggaataaaaacgtggcagtgagtaaccagagaaggccaacacaatgcattttgcatttataaagggatgccagaaatggtggaaaaactacaa
ttcccctttacctttcagcaccatctttgctagaaaattgatgctgggatctgtagttccaccatggatagactgccatgcacctggtaggttgtgaagc
aatgataaagtctgccttattggcctcaggtaatcacagaaacatatgtctatatttgtacattatgaactttaaaaagctgcatgtttgaaaatactta
atttccattaatacttgccctttacttgttatttccatgaacacttggcaatatgtagaatcatcctcccacagcactgtcaaaaagttgcttggatttt
attagtgaaaataaactaagttgttatagatatgaatggcaaacaagtttaatctgtggaatgacacctttgttttgngggttaatgagcattgaatttt
tcatgcagacctgtagagaccatgtgacagcatcaatgacct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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