XB-CLONE-3548039
Clone Name: IMAGE:7716116
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Gene:
mpp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981638 [+]
aatacctcgtctcaggatgtcaatgaagtttgagcgcgcagagaccccgttgtgtgacagttccagtgtgaggactgccctgtcagacctgtatctggag
accctgctgcaaaaccggggctcagccaagggctatcaaaccagtgtcggctctcacacagaggagatttacacaaatggatctgcagcttcagggacag
aggaaaagaagagagtacgacttgttcagtttgaaagagaaaccgaagaacctatgggtattacgctcaaggtaaattcacagcagagctgtatagttgc
tcgcatcttacatggaggattcattcacagacaaggttcacttcatgttggagatgaaattctggaaattaacgggaaaagtgtccgaaatcaaagtgtg
gatcatctgcagagtgttctgaaagaaagcaaagggaccgttgttttaaaagtgtcaccatcacagagcaatcgcaagcccccactccagatgtatgtcc
gagctctgtttgattacaaccctctttctgatacactgataccatgtcgcgaggctggcttggcctttcatactggagatatccttcagatcataaacaa
ggatgattccaattggtggcaaggacgtttacagggtgaggggtcagctgggctgattccctctccagaacttcaggagtggcgtgtggcaagctccagt
acaacaaatcagaatccacagagttgtagtccttttagcaagaagaagaaatgtaaagacaaatacctggcaaagcacagttctatatttgaccagcttg
atgtagtgtcctatgaggaagtagtatctctaccagctttt
3' EST: CX981637 [+]ttggcatcaacacttaggatggcagcttacaacaattacaacaaaatccaccatataataaaaacagtttacatccattacatcctttattccatttacc
cccgtttttgttttgttcccctttctacatgcactctttggcttacatggaatgagaaaatattttactatgacttttcaggagacgagtaatggtgata
aaacacctagtgaaacctttctgcaaagcctgcgggaaacaactataataggggactatctgcccaatttgcagaaagttgcaaaaacatgcctcccata
aggctttgctgagacagaatcccagaaattatagtaaagcctgagttttccggtctcctatttacaaaaccattctcctctggcaaacccagtcctcttt
gggacaatggtaaatagggcgattttgaaatattgtggttttggcttttgccccttgattcatttgaactgaaattttctgaaacggataattttggcca
tcaaaatctccctgcatgccattacccttcatcagtaaaacaaggttttagttttgtattctattgtttgtggtagtcactttaactccaatctgtacat
agaatgtggaataaaatggctcttttgaacatttggtagaaaaatataactggacaggaaggaaaagtaaaaaaataacccaattctgccataactttgc
taatcaacaaactccattatttctataaaataatatcttgataaaaacaagcatgaatttgcttctctgggttaatattgttaagtttttatacaagttt
agtaaagcagctagttagcagcaacctctgatgcaacaaattctcattttggatctgaacatagctcacattaaatgcccccctaatttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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