XB-CLONE-3547997
Clone Name: IMAGE:7716072
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Gene:
aplp2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976959 [+]
tagtttttagaggtttgcctcttaaaaatgagtgtttaatttttgacattacatccncttttaagcaacttttccactaggtttttcaatctggttgttt
tagattcaggttgagaagagctgttgtgtactactgattattttgtagatattttcctgcttgggccagtgcgctctgtgtttgtagaaggcagaacctt
aaagaattgcatccatgtaagatagtgcagtctactcgtggtcttgatgccattaagagtaatttagtaaactgatgcaccatgtgaaattatcagatgg
tattttccattcatcattgctatactggtacctttgcacttaatcctttaattgtcttcatggaagcaagaacaagaatggcagttttttttcccctttg
tatgattgtgtacaacaagcacttgcagctttctattctgtggaactgtctgccagggctaagaccatcaataaactaacatttcatagggaacaagggc
cagtagtactgctgcccgagggactcgtagaacaagggactgatagcttccacttagagtcagagctgtcttcagatccccaccctgtctctgtaatgct
tgtaaatgtgatgtttgctattactgcatcatttcaaacgtaattattttctaaatcttaaggttctggtaacctgtggtgtttgtcttcctatatattt
ttccatatattactgtgagtttttaatttgttttcttttgccaggattttttttcgcactatgcacagatttactttaaacctgcaagattcctttgtgt
ngtctgtggg
3' EST: CX976958 [+]cccagaacaccaaaggaatcttgcaggtttaaagtaaatctgtgcatagtgcgaaaaaaaatcctggcaaaagaaaacaaattaaaaactcacagtaata
tatggaaaaatatataggaagacaaacaccacaggttaccagaaccttaagatttagaaaataattacgtttgaaatgatgcagtaatagcaaacatcac
atttacaagcattacagagacagggtggggatctgaagacagctctgactctaagtggaagctatcagtcccttgttctacgagtccctcgggcagcagt
actactggcccttgttccctatgaaatgttagtttattgatggtcttagccctggcagacagttccacagaatagaaagctgcaagtgcttgttgtacac
aatcatacaaaggggaaaaaaaactgccattcttgttcttgcttccatgaagacaattaaaggattaagtgcaaaggtaccagtatagcaatgatgaatg
gaaaataccatctgataatttcacatggtgcatcagtttactaaattactcttaatggcatcaagaccacgagtagactgcactatcttacatggatgca
attctttaaggttctgccttctacaaacacagagcgcactggcccaagcaggaaaatatctacaaaataatcagtagtacacaacagctcttctcaacct
gaatctaaaacaaccagattgaaaaacctagtggaaaagttgcttanaagggatgtaatgtcaaaaattaaacactcatttttaagaggcaaacctctaa
aaacta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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