XB-CLONE-3547983
Clone Name: IMAGE:7716360
|
Gene:
cct6a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX976971 [+]
tttcagtgccataaaaaatgaataaataaatgttaaacattgaaagttttcaatatttgctatcgttttacagctgtgggagtagattttacagaaagtt
tattaagtaacttcagcaaatcagtgggagcaaagcgcaaataatttcagatttgaataaacttcctaactgaatcataagaaaagtgtcacaacaaggc
tatattcatcctttcagagaagacattcccgctctcataatctcatccaccaacaggatattgcttgcaatcacagtacaggaatgaagcagctgcttct
taacactgtagttatcccaaattcctgcctcagatgaaatcataggttcaccagtgtttaaatcaacaccaatgagctggcttgaatctgcatattctgt
ctgaagttttacaagtgtctcctgggggtcataaccagagttttgggcaagcaccttgggaatgatgagaagtgcatcagcaaaagcctgaacaccaagt
tgggcacgtccttttacattaggcttgtgcttcacaagagcatcagcgattgctacttccagtgcccctgcgcctggaaccactgaacaatcttcaattg
cattctttacagcacgaagtccatcccttatggcatctttaatttgtgttattgtgtgcttatttggccctttgattaatagcgtcacagaccgtgggtt
ctcacactgttctataaaagtgaatttttcttctcccagtgtgtattcatagacaagaccagcatgtcctaagcattcaggtgtgaggtcatccacagaa
ttcatggcattgccaccacaagccaaagtcaatctc
3' EST: CX976970 [+]ggcgagaggctacccccgtcactatggccgcagtcaaagccctcaatccgaaagccgaggtagcccgagcccaggcgggcctggctgtgaacatcagtgc
ggcaagggggctccaggacgtgctccggaccaacctcgggcctaaaggcaccatgaaaatgcttgtttctggtgctggagacatcaagcttaccaaagat
ggcaatgtcttgcttcatgaaatgcaaattcaacatccaacagcctcgctgattgctaaagtagccacagcacaagatgacattactggagatggaacca
catcaaatgttctgatcattggagagctgctgaagcaagcagatctctacatctcagagggtctgcaccccagaatagtgactgaaggttttgaagcagc
aaaggtcaaagcacttgaagttttggagcaagtaaaagtgtccaaagaaatggacagggagactctgattaatgtggcaaggacttcacttcgcaccaag
gttcatgctgaattagccgacattttaactgaagctgttgtggattctgttttggctattcgacaaccaaatgagcctattgatttatacatggttgaaa
ttatggagatgaaacacaaaacagaaagtgatactacgctgataagagggcttgtcttggatcatggagctcgtcatccagatatgaagaagcgtgttga
agatgcttttatcctcacttgcaatgtgtctctggaatatgaaaaaacagaggtgaattctgggttcttctacaaaagtgcagatgaacgtgaaaaactg
gttaaagcagagagaaagtttattgaagagagggtgaataaaatcatagccctaaagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: