XB-CLONE-3547934
Clone Name: IMAGE:7716413
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Gene:
ube2e2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977006 [+]
tacactggatccccctccaaactgcagtgctggacctaaaggagataacatttatgaatggagatcaaccatactagggcctcctggatccgtttatgag
ggaggggtgttctttcttgatatagcattttcaccagattacccctttaagccaccaaaggtgacatttcggacaagaatttaccactgtaacataaaca
gtcagggggtgatctgtctcgacatcctgaaagataactggagcccagcactcacaatttccaaagtcctcctgtctatctgctctctgctcacagactg
caaccctgcggaccctcttgttggaagtatcgccacccagtacatgaccaacagagcagaacatgacagaatggccagacagtggaccaagagatacgct
acataggaatataacaaagtggacctgtgcaagcacattcactaagtgcatcaatagctcttttctttcttttcttttttgtttttgttttttttttttt
tttaattctttaacagtttgtgacaaaatactgtatgtccttttttattaagactttaacttgaatttccctaagaaataactgggaattttagtttctg
tgcaagaaatgttgaaatatgtgatagtatagagctttgtttgaaagcattgtatcaatatttgtgtttcctgttttgtttttatattgtattcctattt
tatgagggggtataatttaatttcaagttgcccctctacatagactagagttagatttgatttttgttacaatttgatctttttgtacttaaagtcttca
tggtgggtgcatgctccaggggacaaacttttatagataacatttgcaatgc
3' EST: CX977005 [+]cctatagggcgagaggcttttttcagtcgcagtgtctttattgagtgctttataacacaagtgtacatcaaatgctccacaccctgctaaattaatttac
aagcattttaaatagttcaaaatacacctttttttttgttcattcagaaagtcctctgtgggagaatcacttccttgttacgcacggagattccgcattg
caaatgttatctataaaagtttgttccctggagcatgcacccaccatgaagactttaagtacaaaaagatcaaattgtaacaaaaatcaaatctaactct
agtctatgtagaggggcaacttgaaattaaattataccccctcataaaataggaatacaatataaaaacaaaacaggaaacacaaatattgatacaatgc
tttcaaacaaagctctatactatcacatatttcaacatttcttgcacagaaactaaaattcccagttatttcttagggaaattcaagttaaagtcttaat
aaaaaaggacatacagtattttgtcacaaactgttaaagaattaaaaaaaaaaaaaaaacaaaaacaaaaaagaaaagaaagaaaagagctattgatgca
cttagtgaatgtgcttgcacaggtccactttgttatattcctatgtagcgtatctcttggtccactgtctggccattctgtcatgttctgctctgttggt
catgtactgggtggcgatacttccaacaagagggtccgcagggttgcagtctgtgagcagagagcagatagacaggaggactttggaaattgtgagtgct
gggctccagttatctttcaggatgtcgagacagatcaccccctgactgtttatgttacagtggtaaattcttgtccgaaatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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