XB-CLONE-3547698
Clone Name: IMAGE:7716387
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Gene:
fbxw2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977163 [+]
aaacagcatcacttattggtcatagcgccagagtttatgcactttattacaaggatggactcctgtgtacaggttcagacgacttatcagcgaaactttg
ggacgtgagcactggacagtgtatatatggtatccagacccacacgtgcgccgcagtgacgttcgatgaacagaagctggtaactggctccttcgataac
actgttgcctgctgggattggagtacgggagccaaaacacagcacttccgaggccataccggtgcagtgttcagtgtggactacaatgatgaactggact
tactggtgagcggttcagcagataacactgtgaaaatatgggccttatcatccggaacgtgtttaaataccctcactggccatactgagtgggttacaaa
ggttgtgttgcagaaatgtgaagtcgagtccatcgtgcacagccctggagacttcattctcctcagcgcagataaatatgagatcaagatctggccaata
ggaagagaaatcaactgcaagtgcttgaagactctctacgtgtcggacgatcgcagtatatgcttacagccccggctccacttcgatgggaaatacattg
tgtgcagctctgccttagggttgtaccagtgggactttgctagctatgagatacagagaatcatcaagatgccagattctaccaacctctccctacttgg
cttcggcgacatctttgctctgctgtttgacaaccataacttatacataatggatttacggacagagaaactgatcagccgctggcctctgccggaatac
agaaagtccaagaggggttccagctttttggcggggggaatagc
3' EST: CX977162 [+]cggatataccctcactgcttattataggctgtcccactagggtattttacagaaccaatgaaaagtctttgacaaccaaaattaaaagaaaaaaaaaaaa
aaaaaaaaaggggaaattgtcctgttaaaatatcagtagataaaataaacaaaagggaacaatgcacatataaatggttaaataaaggtccagtcatgta
gcccataacaacaagctgtcattactgcacccgttagttgctatgggctaccaaactggcccacattttgaaaacatttttgaattaacccctgtatttg
tatttttccccccaattaaagggacagcgttccctacttacaagtaggttatatcttccatttaaaggttcggagaagcagaatacaaagcaacaagaac
gtatcctccttgattaagtcttatgtattgttactacctaagtgctcaaacaggaatactctaagctccggaatactctacctctacgcggtgcattttt
acattgttaaatgcgttagctatttccgtagtaaaatagattacatatataaacaatcctgaaggctgacatatacatagcacggttgtagcatggagag
agcgtgacttttcttttattattgcaacttcctggtcaattttaccagccttttttaaaaaaaaattcctttttatccagaacgcatcaggggtgggcgt
aaggagcggtggaggttaaaaatggcggcgtcttgagtcctatctggcgtagacagatacgtttcagtgtttggtggaacgctacggtgagatcccgccg
gcatgcaatctggttggttcacaaataaaaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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