XB-CLONE-3547675
Clone Name: IMAGE:7716389
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Gene:
gmfg
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977178 [+]
cttgttcacttatataaaaaaatacccaaatcatttgttgttcaataatttgccattgtcttaaaagtattactctgcactaaatgactgacctatttat
taatacttttgtgatatcattcatttttatggaaaagatgtgacaataatctggattaacagagtggcgcagtgaaccatatgaacctgatttgtaaata
acgtttgctctatctgtattctcttccttgtgtttgccatctttctgctctttacttagttgttgcattctctttttgttgtatctctgggtgcattctt
ctgtctttagtagttctctgttgattcccttctatttattattattaataatatttttaatattattaacatgtacttaaaaagtgccaacatattcagc
agtgttgtacgataagtgggtttatacattagacgtacagaattacatacaaagcctgcccaaaagaacttacaatatatttactgctttgcatgcactc
atcaactgttgtaacacaaggataaagaaatatcttgtaaaaatagcatctggcctcttttttatgctgtaaaatcatgcacatactagtcagccttgtt
aacctacgctgggcaatgtgctttgtgcagttacccacagcacagtcttagcatttatctttcatctgtttactgcccatagaattatgaaattaaatct
ctgattactggttactatgggttactgcacctggngaaatttaaccagtgatattacatgagcccactggtttttattcactttttgatcacatacctta
cattctagctttaagcaatcaaagcaagtgaatagctgtatttat
3' EST: CX977177 [+]cctttttttttttttttttttttttttaagaatttctaccttttaattcattttctcaaaacattagctgccagcaatcaccaacaaaaacacaactttc
tcttcttgcttttcctgttcctgtgaactattagaaatgagaactgtataattgaaactcaaagcataaacactgcatgggaagttttttgaatgagctt
ttcagctgttaactgttttagggctagttcacaagctttagagggaaaggagcccacatggtaaaggatatcatgcaatggaaattctcttttgtaatta
agtataacagcttccacctgttggctattaagtataatacctaacaggtgtaaggccatccaaaaataggaatgtctgccatggcttcacatcagcggaa
aaatgccaagcgttcttttagccagtcttctgtcaggtcacttgtgtttctgatctcaaagacctacagtgaaaaaaaggcaaacatttagtgagaagtg
cagaacagggaccttgtactgcaaaaaaagatgcagcaaagatgcttaagcagagcaaccgaatacatttttcagcactgcacatggcacccctacatac
cctgttgtgcctgatgtttattagcctcactcccccatagtatgctacatgaatgccattgtggtaacttacactttcggatcgccaatcgtagacgatc
ttttcaacatgaaaatttctgaactttcggattatataacggaattttcgtgtcttttgcaatcatcagaaattat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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