XB-CLONE-3547614
Clone Name: IMAGE:7716443
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Gene:
avpi1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161573 [+]
tgtctctacgagatgcccatatcgtcgcgttaaatcagtgtcttcctgagtggtcagaaggcattatggggactccggcctccgttgtcagctcaccatc
tgccccctggcaagcaccagtggcaagatcaaggaaaaaggcttctgctaatatatttaaagacataaaccttcttcagatacagactttgttccacacc
agcggggatgaatgcgctgaggagcgagcacggatcatatgtgaatatgcaggggacaagcacgttgcagatgccttgattcggctcagaaggaagaaaa
agagcaaacgagcccacaagccgtcttccaaaccaagcaatgatagaatcgaaactctgtctgtgcaccatttcagtaaactatgtattaatgaagccga
tcgcaaaggaggtccagatactgaggataataaccctgaccaatcaagcagcgctggccaagaaccacagccttgtgagacaaagaaacagacagtaaca
actagaagagggaaaggtaaagtaaggaaagagacacaggaaaggctctcagactatctgcatcagataaagcgatgatctaggacatttaacatgagaa
atacatgacagtgctgctatttattgcaatacaatttctctgccttgggaaaggtgaaataagatattaataaaatactgtacaactaaccataccagtg
caatgaactggcataccagaactgacggactaaatgaacaagccaaagcttccactgaagaatccttacatggccccagctctgtttcatatggactgac
agcccacgggttctagcgctataataggaatgcatgaaatgggcatgataagcgaatcagcatggccatatacagaactaacacataagctggaatagac
cctaatataacagatttggtaaaaacactatttcctttcagttttcataatgatgtttcagacctgtatgagaagtaatatgttctgcagtcagcatgga
acaaagacaaacggacagggccttatacatgtacaatttatttaatgatgctgctttacccccaccaaactgcccccaaggacaactgatgggggtaata
tacagccaagtgaacagcgtgtcttcaaatctttgcccaagcaataagtgttgcatccaactatccatgggcagatgacaaagtaggacatttgtgtgtg
catctctgtaaggttgatcttcaaattctacttctccagctgctgctgggccacagctccctgcatgtcactgatagctggtgaatggcagagggttata
gtccagcaacagatggaaagctgcagattggagatcagatcattgctttaaaataacagaggcaaacattttagtggcaagggatataagatgtagtaaa
agcatctcagaccttggagtcaaatgctgttatatgcactaattatttttctgtaaatctgttacttatctaattgctgcttatttaaaagatttcagtt
cctaagtatacattgcactaaatgtatgattcttggacggaatttgatttaaatcattattcattaacctttattgcacatgcctggttaaggcacaaag
tattcttcctatagggtctctctttactttgctggcggaattcacagaagtgtatatgtagcgccggcactgcaaatccatactaaccaatttgcagtta
accttacagggacactatgagttgctagaggtgtaacattacacctgaatttgtttattctgccctcagtatctgtaaaagagagttgtcacccatgcaa
taagtgccccagtttcactgttacgccaccaacttccagtaataataataacagcatagtgcctttttctttcacaagcacaggacccaatcctttccag
caggctggtgttgttctataggtttctattttttgcactactggtcaaatgttcatagtaggacacaaggcagatttagtagatggacagttttctatgt
aattatgatactgcctgccccttttttctaggacaatgatgtgtatttgagggttagtgtcccctaatgcttggcactgagattggggtaattttctaat
ataggtgcttaactgaaattccccatagaatcaagcagatgtttgtttttattttctaaagctggccatacatgtaaagatacactccttagtaaggttg
gcaaacgagcagacctttcacccataggcctaccacgaggtgggcattatcagactggcccagcggtttcgccctcaggccaacaatccgatcataattg
gagaaggctgtcggttcgagggctgtatctggaaggaaccagtcggcagcttgtatctgcccatgtatggccacctttatttgtagtagatcaagtagga
tcaaagcaaatgcttattgctatggttaaccacactgttgtaattcagtaccagtgttattaaatgaggcctacatgaatgtgtaaactaataaacagca
ggcacaaaaaaacataaaataatatttgctgttttaatatgtttatatggaaagggtattccttcacacactgcctgtcctctaaagcaggaagctctcc
ctgggctcctgttttatgactactataaaatatagctagaaaaactaaaacaaaatgaaaaacagcacatggattcattattttgagttgtgcaaaggga
agaatatgtttaaagacacatgggtagtgatgttgcaatatgtattaaagttcagcaatacttagtaccatttttgcatatgttttcacaaatgtattta
gatactacagttttgtaatgacagatattaaaacactaatctgttgtttggccataaggttaaatttttgcactgaaatgctgtgcctgtccatatgtat
acaaagcagccctatggtccaagcctctaaaaccctagtccctggcatcctgcttcacaaggtagtaagtgctaacaacagaatcggggcaacaatacag
gggctttaattggtgtcaagcttctgttgagaaaaggccaatggcagatcattcaagcactatacttgctttaagaacaaagttgctatagtcctctcag
aggcagagtcaggcagaaaggggcattttgaccgttttgacactatttctggccacagtggcggtcaaaacaacctgttccatagaccttagtgcttctt
tatggtaaagtatttattggattttagaaaatgtacaggaaaaaaagaggtaattctaagacatggtccctattatatttaattctctaggcaataataa
aataagaaaagatcccatgtcttttaaaactaagcaaataagatgaaatttaaggcactgtctgtgtgtgtgtggatgtataaagataatatgtatcaca
atgtttaactgcacgtgacaggaacatttgtaataaactgcataaagcaaaaaaaaaaaaaaaaaa
5' EST: CX977218 [+]tgtctctacgagatgcccatatcgtcgcgttaaatcagtgtcttcctgagtggtcagaaggcattatggggactccggcctccgttgtcagctcaccatc
tgccccctggcaagcaccagtggcaagatcaaggaaaaaggcttctgctaatatatttaaagacataaaccttcttcagatacagactttgttccacacc
agcggggatgaatgcgctgaggagcgagcacggatcatatgtgaatatgcaggggacaagcacgttgcagatgccttgattcggctcagaaggaagaaaa
agagcaaacgagcccacaagccgtcttccaaaccaagcaatgatagaatcgaaactctgtctgtgcaccatttcagtaaactatgtattaatgaagccga
tcgcaaaggaggtccagatactgaggataataaccctgaccaatcaagcagcgctggccaagaaccacagccttgtgagacaaagaaacagacagtaaca
actagaagagggaaaggtaaagtaaggaaagagacacaggaaaggctctcagactatctgcatcagataaagcgatgatctaggacatttaacatgagaa
atacatgacagtgctgctatttattgcaatacaatttctctgccttgggaaaggtgaaataagatattaataaaatactgtacaactaaccataccagtg
caatgaactggcataccagaactgacggactaaatgaacaagccaaagcttccactgaagaatccttacatgg
3' EST: CX977217 [+]gctttatgcagtttattacaaatgttcctgtcacgtgcagttaaacattgtgatacatattatctttatacatccacacacacacagacagtgccttaaa
tttcatcttatttgcttagttttaaaagacatgggatcttttcttattttattattgcctagagaattaaatataatagggaccatgtcttagaattacc
tctttttttcctgtacattttctaaaatccaataaatactttaccataaagaagcactaaggtctatggaacaggttgttttgaccgccactgtggccag
aaatagtgtcaaaacggtcaaaatgcccctttctgcctgactctgcctctgagaggactatagcaactttgttcttaaagcaagtatagtgcttgaatga
tctgccattggccttttctcaacagaagcttgacaccaattaaagcccctgtattgttgccccgattctgttgttagcacttactaccttgtgaagcagg
atgccagggactagggttttagaggcttggaccatagggctgctttgtatacatatggacaggcacagcatttcagtgcaaaaatttaaccttatggcca
aacaacagattagtgttttaatatctgtcattacaaaactgtagtatctaaatacatttgtgaaaacatatgcaaaaatggtactaagtattgctgaact
ttaatacatattgcaacatcactacccatgtgtctttaaacatattcttccctttgcacaactcanaataatgaatccatgtgctgtttttcattttgtt
ttagtttttctagctatattttatagtagtcataaaacaggagcccagggaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: