XB-CLONE-3547571
Clone Name: IMAGE:7716543
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977248 [+]
agctccagaggagccaattaacaatcccgccctccccaaagggagaaactgaaggaatcagtcggcaccagccaatcagattccagcagggtcactcgct
ttgttcttatcctatagatctaaaataatcagcagccccccccaaccttacagacaatatggtagaaccatctatacaccttagaagaattcaagtagtg
tcgggagtctaacgggttaaaaaaacatggcggcgtctcgggagatgccatagtgattgaccaggagaagccagctcccagctcaggtctaatagtcttt
ggtctcgctgccggtctcgttcaagatggtcgttcggaccagcgcctcggtaacggcgtaggggtcgcagtttgctgccggccggcggtcctcgaagtaa
ccgtagccttcctgccccacctggcgggggatccggatgctggcaccccggttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcc
cggtgagtctccgggagttgtccttcccgccccgggggtcatacacgcagatgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtg
tttgagtcccccttcgaccctcatgctctctgtactgtagttggtatggcaaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggcc
accaccccgaagtcctcgcacacccggtggaggatgaacctggccatccataggtggtcccccatgtcgatgccttcgcacggtcccacctggaactccc
actg
3' EST: CX977247 [+]ccagtccagatgttcagagaccccttctgcctggaccccaataagctggttatgtgtgaagtcttaaaatacaaccgcaagtctgcagagaccaacctcc
gacacacgtgtaagaagatcatggaaatggtgactgaccatcgcccatggtttggaatggaacaggaatacaccctgctgggcattaacgggcacccgta
tggttggccagagaacggcttccctgggccacaaggtccctattactgcggcgtgggggcggataaggtgtacgggcgggacatcgtggaggcgcactac
aaggcgtgtctatacgccggcatcaagatctgtggcaccaacgcggaagtcatgccctcccagtgggagttccaggtgggaccgtgcgaaggcatcgaca
tgggggaccacctatggatggccaggttcatcctccaccgggtgtgcgaggacttcggggtggtggccacgctggaccccaagcccatgaccggaaactg
gaacggcgctggttgccataccaactacagtacagagagcatgagggtcgaagggggactcaaacacatagaagatgccatcgagaagctcgggaagagg
cacaactaccacatctgcgtgtatgacccccggggcgggaaggacaactcccggagactcaccgggcagcacgagacgtccagtatccacgagttctcgg
ccggagtcgccaaccggggtgccagcatccggatcccccgccaggtggggcaggaaggctacggttacttcgaggaccgccggccggcagcaaactgcga
cccctacgccgttaccgaggcgctggtccgaacgaccatcttgaacgagaccggcagcgagaccaaagactattagacctgagctgggagctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: