XB-CLONE-3547544
Clone Name: IMAGE:7716593
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Gene:
casp2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977266 [+]
catcagcaacgtcgcatttgagacccaagatcttgatcacagatacggcggggaagtcgatgtcacctctctggaaaagttgttcagcagtttgggcttt
caagtggaagttagacgtaaccttaatgcacagaacatgatgtcccaacttggagccttctctgcactgccagctcacagcgctctggactcctgtgtcg
ttgcagttctttcccatgggctcgatggtgctgtgtacgggacagatgggaaacttgtccagctgcaggacgtattcacagcgatggacaacgctcactg
cccgcagctgcagaacaagcccaagatgtttttcattcaggcctgccgaggagaggaggctgaccgtggggtggatcagcgggatgggagagagcagtcg
gcttctccaggctgcgagcagagcgatgccggcagggaggacataaaagtccggctccccacacagtcagacatgatctgtgcctatgcctgtctcaaag
gcaccgtgtccttgcgtaacaccaagcgaggttcctggtttgtgcaggatctcgtctctgtgttctcccagtactccaaaaacactcatgtagccgacat
gttggtcaaggtgaatgcacttataaaggagcgcgaggggcatgctcccggcactgagttccaccggtgcaaagagatgtcagaatattgcagcacattg
tgccgtgacctgtatctcttccctggcattgggccccccaagtgattcccccggcattactgcccaggaaccagccacttcttttgtacatttttttttt
tatgtaatataaatggtttatttttaca
3' EST: CX977265 [+]cttccatggtataatggcgagacactctactgcaacaggtcccgcagtgctacgacagagtgattggccaagtataaaatttacagtacgtggcagtgac
aaataacgagacagacctgagagagagtcaaagtgcagcaaaatcatgatactggcaggacgtcgggcagttacaatacacagagaaacgtaggggtgtc
ccaaaagttcttcaggagtctacctcctggagtattatactgccataggaaatggatctttgaaaattacagaatacacacggctgacattaggttgagt
ggatccttgtttaaaaaaacaaacaaaccactggcttgatctgaagggatcctggaacagaagaaatcctctggattagagaatacacatctccggcttt
gggctgaggggatcttgggatagcggaatacatgcctccagctttgggctgaggggatcttgggatagcagaatacatgcctctagctttgggctgaggg
gatcttgggatagcggaatacacacctccggctttgggctgagggggtcttgggatagcggaatacatgcctctagcttagggctgaggggatcttggga
tagcggaatacacgcctctagctttgggctgaggggatcttgggatagcggaatacacgcctctagctttgggctgaggggttcttgggatagcggaata
catgcctctagctttgggctgaggggatcttgggatagcggaatacatgcctctagctttgggctgaggggatcttgggataacggaatacaggcctcta
gctttgggctgaggggatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: