XB-CLONE-3547525
Clone Name: IMAGE:7716595
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Gene:
pnpla6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977278 [+]
ctgcctaggagagcgagagacggctctccacctgtccccccgcatcagaacctcctatatcaacggttcttctgctttgtgtggttctagatatagatct
tccggactgtagcggtttgtggcccggccacgtacagacgcaataaagcctgtggctcgcgagggcgcgtcacgccagagtatttgttccgttcggaggg
agccaaaaatctattcaagccttaaagcataatgtgcatttctttgcttagagtaagagataacttatatacaggagatgcttggtccactgggccatac
cattattatagagtagcatcgaccactgggccataccattattatgaagaagcatccagcactgggccataccattattatagagtagcatcgaccactg
ggccataccattattatgaagaagcatccagcactgggccataccattattatagagtagcatcgaccactgggccatatcattattataaagaagcatc
cagcactgggccgtaccattattatagagtagcatcaaccactgggccatatcattattagaaagaagcatccagcactgggccgtaccattattataga
gtagcatcaaccactgggccatatcattattagaaagaagcatctagcactgggccgtaccattattatagagtagcatcgaccactgggccatatcatt
attagaaagtggcatccagcactggatcataccattattatagagtagcatctaccactgggccatatcattattagaaagtggcatccagcactggatc
ataccatttattattgagtggcatctatctgtgggtcatattattgccattagtcatgccattattat
3' EST: CX977277 [+]ggtgtcaaacgccttttattgtagaaaacctgttagtacatgagcagtcgccactgtttcctaaatgcaccccaaggcaagatgccccactggcaataag
gtcgggggcaggtagtgggggcatcagggccaggcagcagtggcagcagcaagaaactcaatgttccagtgaaacccggtgcagcagaagccccagcagc
aatgggaggagtcactgagccaaagcttggtgctgattgggtggagttggccgattatggcacagaggctcatgggaaggggatgattgtcagcctgggg
ccctccagcttcatactaacaagtgaggagatacaggttcaaccttagtattaagtcccaccagggagtagccacgccccacagacccccccccaatgcc
aggctgctgggagtccggcccagaccagggacattcaagtcacaaaacaaacaaacaaaaaaatcataattttataataaaaatgttaaagcaaaaacgt
atttaacgttactttggggatccagcgggggaggggagcagcttactgggagttaggggccctggggaaggataaagtgactggggccccccgtgctaca
gtgccatgctgtaatgtagaatgtaacaaacccacagcattaaccgtataataaccctaatgccccctttattggtagggccagagccagatatagcccc
ccccccggctatggggtaaaagcaggtatcagcggggccacagtgactaattccagtataatcccataagcagtgtcacgctgcacaggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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