XB-CLONE-3547488
Clone Name: IMAGE:7716503
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977304 [+]
cacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggcagcagaattataaataaaatgg
aacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagt
gtgcatttacttcacttttacattttcttcttacttgatttgcagtaggccgacagcccaatctctgggtatttttttttctttttatgattgatatggc
atacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaag
tcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctct
gcactagtgccttgttgagtgcctctccatagggagtgatgctaactttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatgg
ttctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgt
tttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggctaaattctcctgcatctcctctgcataagggc
tcaggctctc
3' EST: CX977303 [+]gccatcacaggtatgtaatgatgtaaaaatataaacaaaggaagatgatagaggagggtcacagaaaccaaataacattccaaataaagtaactggtatg
gtggagatttatatatgggacatgaagagcctaggttctaggcatctagacaatatgctttgtgaaataaaaaacactcccgaaacacttccgaaacata
atgcaagtatatgtgtactgcacaaatggtattgtatgtcatcttaccactttctcttcttaggctcccaagccgatgtcagaacagaccaagcagtcag
tgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggaccagatccagcagtcagatatcaccaaacaacttaatgttctcata
caggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattccctttgcaaagcaaatccatgaccaactaacacaagattcag
agaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttgga
ggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaa
gaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccatattcagaanaactccaggcccaaatagatgagagtgtagacc
aactaagaaaacaacttaacccttacactgatgaagtgagagataagattggcccatcagtacaaaagttacgtgagagcctgagcccttatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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