XB-CLONE-3547433
Clone Name: IMAGE:7716510
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977343 [+]
aaaaatatgtctcaagttactttaggaaggaatatttatgaacatggcagtatattaaaaaaagttattgtgacatgggatgttgggatgtttggagtca
ctaacggccccttctaaaccctccacttctatttctgcctcttcctcctcctcctcttcctcctctggaattatttcttctatcaaaaggtctcctccct
cttcctccaaaacctccattccttggaccatcaaagtttctctcagattcttgaatcgcgggtaattcagttgcaactgtaaactgccaccgccttgtat
ctttccagccctcctgcattgactgcaggttttcagaacggacatcaaaacacacacccatggaatccttcaggagacacattctatgaattttagaatc
aatatcttcacccagttgttctttaattgatcgccatgcatagcttaagttgtgaatttgcactgagctcttcaacgttattgtcacataacccgcttcc
atgttgagcaaagagcgttgtttaatggacgtcgccccagaaatatgagctaaagctgcagctagggcagttaatgcacccttcttctcaatcagctcct
gggcgtattctttaaaatgctcaatcacatcagctggaactgaatccaaggactttattgcatcagcactggatgatttggcaacattcaataaggaagg
tattccaacacgtttgaatgtgattcccgtgctcctctccacattccgaagataatgcctttctcttggttcataaagggatatgcagattcctgtgcgt
ccggctcttcctgtgcgccctgaccgatgaacataagcatctgcttcctttggtgcagagtacaatactacaaggtcaacttctggg
3' EST: CX977342 [+]ctcctatagggcgagaggctttatgccgatgaaatggaggttgaaagtgtcaagaagaagaaacagtctgaaactcctctacccaagataaaaaagagaa
aaatgcagaatggggcaacagaagatcttgatttggaacaggtggcagaatctgtaaatggagagatcaacaacaataatccgactcccaagctgaagaa
aaagaagaagcctgtgccacaatcagatctctctgaaaccgcagaacagtatgaaggagagcagccagatccaagcactcccataccaaagaaagtcaag
aagaaaaaactaaaagagggcaaggaagattcagacacacaggaagggacagacaaatccgaaccacaaatgaacggcgtaaagagtgttaaaaaatcta
aaaagaacacaacaggtgatgataatgaaccaactcctaagaaaagaaaaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcagtt
aaccaaagaggaagaggaaataaatcaagaaaaaatagatggcgacttctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaaggc
gtgtcctacctgttcccaattcaatccaaaacattccacactgcttacagtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacatttt
cctttgctattcctttagtggagcggcttaatgaggaccaacagccgttggctagaggacgtgcgcctagggtgataattcttactcccaccagagagtt
agctattcagatcacaaatganatccgcagcatcactaanaagctgaaggtctgttgtttctacggaggaactccatatcaacagcaagttttctccatt
aaaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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