XB-CLONE-3547149
Clone Name: IMAGE:7716536
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Gene:
hnrnpa3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977532 [+]
agcttaaacaggaggcatttcttgttcaggactgtcatagcctcagtttgctaaaagtgcagctgatgattaatgcaatgtagtgttgatttagatgtac
attcctgaggtttttttttttctagttcgtcacccatattccccattacatcaggtataactgccctgtaaattgtgttagtggtaccaggaataaagaa
ttaaggactttttaacttttaaacttgtgtagtacagtgttttctcacttgtcagggcataaagcattgcccaacaaaataaacatgctttcttttattc
ctaatggagttagtgtcagactttcttaagtatcccctactactgagagaatttaaagtgcaattcaaagaaattcaatgccagttttcaaacaggcctt
ttttgtaatataagctgttgcaaagattcgctagtttaacctcacaatcagtggctactgctgaacagtaattgattaaaggggatttcatgcctaaaca
agataatgggagcctaagttatttctctcattaagaacacttttgtaggtttagattcagcttgtttccaatgaatatattgctggtaatggccatggta
caccatgtttgctgcctcaaaatctgatgctagtaaatttagttgccagtgagattatattggctatcaagaaaaaggcaatctttcaccaaaatctatt
agggctcatcacaaagtgaaatgtgtactaacatgcacattaaggtggcttatttcctttgtttcagttagcttcaactctagcactgtccatgacgcgt
aggagcaaaactcttgnttgcttgattg
3' EST: CX977531 [+]cctatagggcgagaggcttagtactcaacacaccttgtaccaatatcgtaagcataatcgtgtatttacagaaactcgacacatggggtctaggtgcagt
ggcaaagctgtggtctgggatgaatccagatataaatcagaacgtactattaaatgataaattatgctggtcatatgatctggtatgatcgatggtgata
ctggacttgagtttcattaaacaaacatctacgattatagcatcatggttatcaattcaatacataatgtgtagccctttcggtagtaggagccatgtgc
cactcccatgcccactcttcagtcacgtcgccaacttggtgtcgagtcagtttcttaagacaaaacgccaggaggaataggtatcaagtgccagttcttg
ctgcagacttccttccacgggaaagccagtttgaagattttcaattcagatcaagcacggtcttatagactcttcctgtcattatcacccctatactgag
ggcccttgcttttaggtctctgtgctagacctattggcttttggtcacacctttagtagctatcattctagtagactccaaaagatgttgcactcttgct
agactgtcttcgttatttcagctgtaactagccttgagaagctgctcactatcagcaaagtaaatgctgctttcacttctcaggaacccgaagctgttgc
tgctttgttactcctttataaaggctgactgggtccatacagtaaaggctgcagaagtcctgtgctagagaagtctcatttaatccacttatctataana
ggttacatctcagtttctttatcattaacgtgtacacaaacatctggtttgtccataaaccctacaaccaggatgtttataaagtctttataagtacatg
caaaattgggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: