XB-CLONE-3547073
Clone Name: IMAGE:7716734
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Gene:
shmt2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977582 [+]
cttggcacagcccctgcggagttgctgccatgttcgcagtcagcacagccaagcttggacacaggctggaaaccaagtctggactggacaggaaagtctg
gcagagggtgaccctgagatgtgggaccttgtacagaaagagaaggataggcaatgccgagggctggagatgattgcctcagagaatttctgtagccggg
cagccttggaagcccttggttcctgcctcaacaacaagtattctgagggctacccagggaagagatattatgggggtgcagaggttgtggacaagattga
attactgtgccagcagcgggctttggatgcctttgacttgaatccagaaaaatggggagttaatgtacagccatattcaggctctccagccaactttgca
gcatacacagctgttctacagccacacgatcgcatcatggggctagaccttcctgatggaggacatctgacccacgggtacatgtctgatgtgaagcgca
tttctgctacatctatatactttgaatccatgccttataagttaaatccagcaacagggttaattgattatgatcagctggagatgactgctcgcctctt
ccgaccaaagcttatcattgctggaaccagtgcctatgcacggctaattgactatgccaggatgagaaaggtatgtgatgaaatgaaagcctaccttctg
gctgatatggctcatatcagtggacttgtagcagctggcgtgatcccatctccctttgaacatgctgatattgtcacctcaacaacacacaagactttgc
ga
3' EST: CX977581 [+]cgctcctatagggcgagaggcttttttttttcactgtaccatgatgtttttgtttattgctctaacagtctctggagtctacctgaatatgctctgctgc
agcatgaatccccttaccccacactgaaatgtcagatttggagttttgagatttcattatggaagtccaatgcaccacggaaatgacaatgaaacaaatc
tagctgcctagttcttcatgcagtgatgctgctggagagaaggtttgccaagtagaaaagggtcctgggtcactccgttataagaaaagataagagacaa
ctaaatatatgatgcaggaatagaatgcaataagaaattggctggattaacagaaaatgcaatgaggcaacaggaagcgcaggaagaattagggtggaat
ggtttgttgttcttaacaccatcaatcatctttttattttattcaacgctcatcaaatccaggcatgggaaaagaacgggcaaactgctccacttgctta
cgcaggtctgctatccgattaactgtttcgtggtcctcaagaagaaagttcttaaagtcttgaagtttgtttgttttgcgcttaacatcaagtccgatgc
ggataccttcatcaatgaaatgaaccactttttcaaagtcggcttccttgaaattccgagatgtcagcgcaggggcacctagccgtaaccctcctggggt
aagtgcgctcttgtcccccgggcatgtgttcttgtttgcggttattgacacaagctccagcaccctctctgctcttgcgccatcaatgccccttgggtct
caggtctactaaaaccaggtgattatctgtccctcctgacaccgtgtgtaacccttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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