XB-CLONE-3546978
Clone Name: IMAGE:7716552
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Gene:
grsf1
,
timm10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977646
[+]
cgacggctcatttcagaaagactggtatccgcccttaccagagtatgatcctcaaccgctagccaataaattaaccaccatgttcattgtgcgcgttcgg
ggtttgccatggtcttgcactgcagatgacgtgctgaacttttttggtgattccaacgtaagaaatggcacagagggagtgcacttcattttcaatagag
atggcaagccgagaggggacgctgtcattgagtttgagtccgctgaggatgtacagaaagcagtggagcagcataagaagtacatgggccaacgatatgt
ggaagtatttgagatgaaccagaaggaggcagagtcgctgctgaaccgaatgcattctgcattgtcaccaaccaggccatcctctatgtccctgtctcca
cagtcttcaatggcctccccaccaagtgatggcgtggtgcgattgcgcgggctcccatacagctgcagcgagcaggacataatacattttttttcaggtt
tggacatagcagacgagggaataacatttgtcttggaccaacgagggcggaaatctggggaagcatttgtacagtttctatcacaagaacacgcagacca
ggcacttcttaaacacaagcaggaaataggcagcaggtacattgagatatttccaagcaggaggaatgaaattcagactgcccgtttcccctttagaaga
aggaaaggagtgacatttgcacccaccataaaagatctttacgatccagacaactgcataaataatactagtaaagacttgctatctgacgtccctgaaa
atggtcacataaatgattacgtcaaggagatgtctgctaagtccatggatgtccaggactttacgttatgtcccca
3' EST: CX977645
[+]cgaaaatcaaaatatgttaggaacaaacccaccacaagtttaccaatttaatgaagaggaaatcaaacactttccttctggaagactaacaatgaacgac
aacggttcactgcacaactcaccagctagtagtgttttaccgttcagtgacgctatctatccgttctgccttctgcattttgaacatgattttatattta
catgacgtacacgcaaaaacctactcggaggccttgcatcacaaaccacattcttattaacttaggcactttgatacaaaaaaaaaacaaaaaaaagtga
taaattgctggttcacaaaatgagaaaggactaaattggcgacagaggaaaagaacattttgcaccagttaattgcagtcaaatctgcattagccaagga
gaaatatctgatccctgctggattggcattcttagaaaccagcaggcacaaaaaaaaaaaaaaaacaacaaaaatgtattagaaagataccagtttgtta
gaaacaaacttctgttgaatggctgtgtaaggaggatgcacactggctaatgtccattggaggtaaaggattgttgctttgtcctttctaagaaagatgt
gtgcaacataatatcaaacactgctctcatttactttctggagatgaattcagataaagttctaaatacccatgttgagtatggcatctgtttttagcca
tggctgctacagcgtcctcatgagtcaagaacctcacaaccgcttctccagtagcgccaccagcgtctgcgctgtactcaatgctgatcttcaaaggcat
tatcggatggaaaaatttngcatatcctgccctgaagcgtgnaacgggaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: