XB-CLONE-3546938
Clone Name: IMAGE:7716795
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Gene:
ctss
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977672 [+]
caagagtctaaaatttttaagaataccctcttgcctatgttgcagcaaatgcccccagataaacccaaataccaatagttttacagtaggtgtagcacta
aaaaaataacatttaatagcccttaaaagcccagtgcatataatgtagccttagtcactaacaaaggcccagtttcagtgtgtatcagtatttgttgtcc
cctggctctgtgcctccgcgaggcttccggtgaatgttagctgttttgctgtaaatacaaatgtataactagtgatgagtgaacctgtcccattttgctt
ctctgaaaaattcgtgaaacggcaaaaagacgcaaaatgcattaaaagtcaatgggtgtttttttaaactactatttttttttcatccattggagtctat
gggcatttttcttggttaaaacctggccaaggacactattgggcgacctctaagaggtcatatcaagttaatcaaaggatcagttattttatatgaatat
gtgcaagtgtgccctgtcgctgtttcattaggtctaagatataaaagtaaaagtgagcaaagatcctttattgctccttattgtggttctggcaagcacc
cttatatttccgggtagcatgtgtaactagccactccacacagattgcctttgttccttgcaatgcggacgtatccttggtctccatactttgtacccca
gctgttttttagaagccagaaatcctgtccattgagatttccatagccaactgcaaggacgccatggttaacttcatgagagcaggttggatcactgtag
acaccacttctgtaaagaaagaaagaagggcgggtcccatctatagncactg
3' EST: CX977671 [+]aagcataaaaaagggggtcgcaaagagtagccggcagagtaaggatgaaatcccttatttttctggcgactgttttggcagccatagtcaaagctcggat
taaccctgcacttgataaccactggctgctgtggaagaacacacataacaaagattatgaagatgagatagaggacctccaaagacgaattacttgggaa
aagaaccttaatttggtcaatatgcataacctggagtattcgatgggcatgcacacctatgagctgggaatgaaccatcttgccgatatgactagtgagg
aaatcaagtctaagctgactggcttaatactacctccccaaagtgagaggcaggccacatttagtagccaaaagaatagcacttttggaggtaaagtacc
agactccatcgattggagggataaaggatgcgttagtgatgtaaaaaaccagggaggatgtggttcttgctgggcctttagtgcagttggggccttggag
ggacagctgatgcttaaaacaggaaaactggtttctctcagcccacagaaccttgtagactgttcatcaaaatatgggaacaagggctgcggcgggggct
ttatgacgcaagctttccagtatgtcattgacaataaaggcattgattctgatagctattatccttatcatgccatggatgaaaagtgtcattatgatcc
tactggcaaagcttccacatgtgctaaatacacagagattgttcctggcacagaagacaaccctcagcaagccctaggcagtattgggtcaatatcagtt
gctatagatgggacccgccctttcttctttctttacagaagtggtgtctacagtgatccaacctgctctcatgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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