XB-CLONE-3546902
Clone Name: IMAGE:7717037
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Gene:
sult1c1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977696 [+]
atcgattcaattcggcaccaggtatggatccagcagtgatgaaacaactggcaaaggaaatggagaattaccaggttattatgggccaagttgagggggt
ccccctgccggaccccacctgtgacgcgtgggactctatatacaatttccaggcccggggggacgacattctcatcgctacctaccccaaagcagggaca
acgtggatgcaggagatcgtggatctgatattacaggagggagatgcgcagaagggcaggagggccccgacttacatcaaagtgccattcatagaattga
tatccccaaagcccatgccgtcaggtgtggagttggcacaaaccatgaaatccccccgtgtcctaaaaactcacttacctattaacctgctgcccccctc
cttttgggagaagaatgtcaaggctgtctatgttgccagaaatgccaaggactgtatggtttcttattattactttcataagatgaatatatttttgccg
gaccccggaacctgggagaatttcttctcgacattcctgtctggagatgtgccatggggcagttggtttgaccatgtgattggctgggggaaagctatgg
acaaacaccagattctctttatcttctatgaggacatgatcgaggacccaatgcgtgagattcgcaaagtcacgaagttcctcgggaaggacttatctga
tgaggttctggagaacatcaagtatcacacttccttccaggcaatgaaggagaaccctatgactaataacagcacagttcccaattctatcatggatgaa
accatatctcctttcctgagaaagggaccgtgggggattggaaaaccatttcacggtgg
3' EST: CX977695 [+]ttcagtagaaaaatatcagacaatgggtttgaggcttcacttatctaagacacaataggcagatctatactttggcccaacaggttccttatggcccgta
cagaggcacaaaggcgccaccaggaaagggagtcgtaggagaatctcttattgggctccatgtgcctctggctcctctgtacaatcttgtgggcacaggt
tgtcccctgtgtgaagatgaggaagggaattcccaccggctccttaggccaaattctctatcagagttcagtgcggaagttcagcccactgccctccatc
ttcttcttgtattcctcatcaaagataatgttctgtgccaccgtgaaatggtttttccaatcccccacggtcccttttctcaggaaaggagatatggttt
catccatgatagaattgggaactgtgctgttattagtcatagggttctccttcattgcctggaaggaagtgtgatacttgatgttctccagaacctcatc
agataagtccttcccgaggaacttcgtgactttgcgaatctcacgcattgggtcctcgatcatgtcctcatagaagataaagagaatctggtgtttgtcc
atagctttcccccagccaatcacatggtcaaaccaactgccccatggcacatctccagacaggaatgtcgagaagaaattctcccaggttccggggtccg
gcaaaaatatattcatcttatgaaagtaataataagaaaccatacagtccttggcatttctggcaacatagacagccttgacattcttctcccaaaaagg
aggggggcagcaggttaataggtaagtgagtttttaggacacggggggat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: