XB-CLONE-3546814
Clone Name: IMAGE:7717045
|
Gene:
id3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX977757 [+]
caagatccagaagaattttcttgaacgctgttttgtgaagcctacaggaatcgaaaacatccagagatttgatctcgatccacctcttaaaactttagca
accatctctgacgccaccatgaaagccatcagcccagtgaggtccatgtcaagctgctaccaggctgtctgttgcttgtctgagcagagtctaagcattg
ccaggggcagcagccataagggaccaggtgtggatgagcccatgggacttctgtacgatatgaacggctgctactccaaactaaaagagttggtgccagg
gatcccccaaggaagcaagctcagccaagttgagattctgcagcatgtcatcgactacatctttgacctgcagatagtcctgggggaagatcagcaacaa
aacagcatccttaacttgcagaaatctgatttctcagaattggcaacacaaggggacgccagtgtctgccactgacctcttctaacctctctggtgtggg
aaactctgagagaggaataaatcctcccatttccatgaagcagaagaattcagattgcaagactatttaaccctttgggacccagacagacctacagcat
ctcatcatttctgatcatcactttgaactttcatccttcactttaaacaagaaaaaaacacaacaattttatggacgagatataatttactaattccatt
taatttcactgggaatcattttaatgaaattcctctgagacctccaacattgattccagtgtttgctagcaaatatcttaaagacttttatttccatgaa
tttttaaaggcttaattattgctaagccgatgcct
3' EST: CX977756 [+]aaaataacaacttttataaaaagttttgtgtgttttttcttcaatcagaaactgtgcagttccaactcagtcttctctactttagagagtgggatattct
ggggtcagagccacagattaaggctttcatgttcgaacatagctagatacatgaaaggctattttatacaaaaatatatatacagctcttattaacaaag
agtaaaaacaagggttaaacagaaagcggaatattttcagtcatggatatttaactaggactttagaaatatagaaaatggctaaaaatggaattgtttg
gagttatttttggtccctagaaggaatgtcaccattacagtaagttacagttgcctgttacattaaggcatcggcttagcaataattaagcctttaaaaa
ttcatggaaataaaagtctttaagatatttgctagcaaacactggaatcaatgttggaggtctcagaggaatttcattaaaatgattcccagtgaaatta
aatggaattagtaaattatatctcgtccataaaattgttgtgtttttttcttgtttaaagtgaaggatgaaagttcaaagtgatgatcagaaatgatgag
atgctgtaggtctgtctgggtcccaaagggttaaatagtcttgcaatctgaattcttctgcttcatggaaatgggaggatttattcctctctcagagttt
cccacaccagagaggttagaagaagtcagtggcagacactggcgtccccttgtngtgcccattctgagaaatcagatttctgnncagtaggatgctgttt
tgtgctgatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: