XB-CLONE-3546727
Clone Name: IMAGE:7716863
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Gene:
hpn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977820 [+]
cgcttccatctcccatcgccagacatggtggagaaagagggcatgggcaaaggctgccgctggacctcgcagaggattctgggagtgacgctctgtgttc
taatctcattggctggaatcggggtcatagcttgggctgtagtgacgtacgttttgaagaacgaaaaccagaacttatacatagtccaggtaaacctgcc
ggaccaccgactgtcagtatatgatgagaaggagaaggtatggcgtctggtctgctcttccttctctaacccagaggtggcgacgttgagctgtgaagaa
atgggcttcatcaggtatggaaaataacttggacaataagagacacttctatcttcaacttgctgctctgtatcattacttgcaaggggattgttagctc
tggcccttggatgatgggcactagctagggccaagttcatattctcatcatttccagggtgtaatcactcatggcaaggccggcatggcagcagaatcaa
tgtggctttttgtttcagtgaaatgtgtaacgtgatgatctacatgagccttcagcgggacctggagctgccaaatggctgaataaagctctttgggatg
gaagaaaggggaaataaaaccacctcttggcttctaatgttttcagactgtaggacgtttctttttgtattggtgcaatggaatatcccatatagaanag
catacgtgtacataggctaaagctaacaggcctattgagtggacagaatatacattgccaatacaatggttccccttactgccttgggccagtgggatga
aattagtgaaactacag
3' EST: CX977819 [+]ctgagctttttttctattggctgtatttcatatcatcatttttcactttttcttgttttggggaatctgagtacctatatgccccctttttctgccccct
atgctcagtagggttgctacgttttccttctgtagtttcactaatttcatcccacctggccaaggcagtaaggggaaccattgtattggcaatgtatatt
ctgtccactcaataggcctgttagctttagcctatgtacacgtatgcttttctatatgggatattccattgcaccaatacaaaaagaaacgtcctacagt
ctgaaaacattagaagccaagaggtggttttatttcccctttcttccatcccaaagagctttattcagccatttggcagctccaggtcccgctgaaggct
catgtagatcatcacgttacacatttcactgaaacaaaaagccacattgattctgctgccatgccggccttgccatgagtgattacaccctggaaatgat
gagaatatgaacttggccctagctagtgcccatcatccaagggccagagctaacaatccccttgcaagtaatgatacagagcagcaagttgaagatagaa
gtgtctcttattgtccaagttattttccatacctgatgaagcccatttcttcacagctcaacgtcgccacctctgggttagagaaggaagagcagaccag
acgccataccttctccttctcatcatatactgacagtcggtggtccggcaggtttacctggactatgtataagttctggttttcggtcttcaaaacgtac
gtcactacagcccaagctatga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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