XB-CLONE-3546673
Clone Name: IMAGE:7717059
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Gene:
cldn8.4
,
cldn8.1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160598 [+]
gtactgctgagctccatctgggaagacaatatggctttgcaacttgttggtctggtgttgggaggcattggcttgataggaacttgtgctgtcaccggca
tgccccaatggagagtgactgccttcattgataacaacattgtggtgtttgaggcacagtgggaaggattgtggatgaactgtgtcaggcaagccaacat
caggatgcagtgcaaggtatatgattcactgttggcactgacacctgacttgcaggctggcagagcactgatgtgcgtggctgtctgcttgacattcctt
tctttcatgatcgccatcattggcatgaaatgcaccgtgtgtgttggagataatgcaagaacaaaagggattatacttctggtagctggcataacattca
tcctttctgggattgtggttctgattccagtatcctggactggtaatcagataatcagggacttctacaatcctcttgttctttcatcgcagaagagaga
gctgggtgatgctctgtacattggctggaccacagcattggtacttattgctggagggctaattctttgctgtacattccggagcggtgaaaaagaagtc
agatacagcctgcctcctaaatcggtaaccagtgctccaccgcctaagtcagcaatcagtgttccaatacggaagccatcaagcctctactccaaaagcc
aatatgtgtaggaaggactacctagagctggagatcccctgtagacaaagtaggacttgcaaaggactgactttctttggactacataatgggagagtaa
gagtgaaccatttgagaatgtgtacagtggtcttaacacaggacttgtggttttggggaacaaataccaagaatcatgagcttatggacatttgcagcta
acaggaaaaaggattttttgtttttttttctgcaccagaatatttcctttcaactgccatgcaagtaacaccgggtgttgaaggaggatcacagactgaa
gacaaagtgactttaactcaactcttgatcttcggtacaattgtaagctctgagggtaacctacaaaatgaagccatcccatgggaatggccgttgaaca
tctgcggtagggcagggcccaagcaggttttctgtatgtgcattgtaaggtagcgtgaattcttataacactcattatgcaatttaataattagcactgc
agggcacccattcctgcagctgccaatgttccctatttatagcgtttactttacacttttactgttttccctgaaatgaattgttaggagcttatcattt
ttccttcaaattatactttaaaatgattttctgagcattagctaagccagcctttctataattttgtagatttttgtagaagtaaagagtgataatggtc
taaatttttatttcaacccaaaacatcagaggaacaccaggccaaaccttagtgctgccttttggtgactttgaataaaagacttttgttgcccctttgc
ccatcccagccgtagacttgccatccccaaaccattgagttcccagggtaaaaaaaaaatcacacagttttattagtaggtgctcttgtgacttgaaggc
aatattatgaacggcatagtggggagttattttcaatttcctatatatttttttttataattgtatataaaaatgcattagtatatacaggcaggggcat
tactgctgtttagattattattgtaacacaggcacacaatatacaatttactattgtattataatataatgcaaacacttataattggttaataacaata
agtcttcaggttccaggatacattggattcaaactctgtctgcagtgaattaggctgtagcaaatgctttatattggaaactacccaagacttataacca
ttcctatttaacggagccctcgggtgttgctgtatacaatgctgttttgaaatatctatacatccagttaagctaaatagtcagtcttcctatattaatg
taaatgtgttgaaactgtataaataaagaataataaataataaaaacccttataacctcaaa
5' EST: CX977856 [+]actgctgagctccatctgggaagacaatatggctttgcaacttgttggtctggtgttgggaggcattggcttgataggaacttgtgctgtcaccggcatg
ccccaatggagagtgactgccttcattgataacaacattgtggtgtttgaggcacagtgggaaggattgtggatgaactgtgtcaggcaagccaacatca
ggatgcagtgcaaggtatatgattcactgttggcactgacacctgacttgcaggctggcagagcactgatgtgcgtggctgtctgcttgacattcctttc
tttcatgatcgccatcattggcatgaaatgcaccgtgtgtgttggagataatgcaagaacaaaagggattatacttctggtagctggcataacattcatc
ctttctgggattgtggttctgattccagtatcctggactggtaatcagataatcagggacttctacaatcctcttgttctttcatcgcagaagagagagc
tgggtgatgctctgtacattggctggaccacagcattggtacttattgctggagggctaattctttgctgtacattccggagcggtgaaaaagaagtcag
atacagcctgcctcctaaatcggtaaccagtgctccaccgcctaagtcagcaatcagtgttccaatacggaagccatcaagcctctactccaaaagccaa
tatgtgtaggaaggactacctagagctggagatcccctgtagacaaagtaggacttgcaaaggactgactttctttggactacataatgngagagtaaga
gtgaaccatttgagaatgtgtacagtggtcttacacaggacttgtggtttttggggaacaat
3' EST: CX977855 [+]tttgaggttataagggtttttattatttattattctttatttatacagtttcaacacatttacattaatataggaagactgactatttagcttaactgga
tgtatagatatttcaaaacagcattgtatacagcaacacccgagggctccgttaaataggaatggttataagtcttgggtagtttccaatataaagcatt
tgctacagcctaattcactgcagacagagtttgaatccaatgtatcctggaacctgaagacttattgttattaaccaattataagtgtttgcattatatt
ataatacaatagtaaattgtatattgtgtgcctgtgttacaataataatctaaacagcagtaatgcccctgcctgtatatactaatgcatttttatatac
aattataaaaaaaaatatataggaaattgaaaataactccccactatgccgttcataatattgccttcaagtcacaagagcacctactaataaaactgtg
tgattttttttttaccctgggaactcaatggtttggggatggcaagtctacggctgggatgggcaaaggggcaacaaaagtcttttattcaaagtcacca
aaaggcagcactaaggtttggcctggtgttcctctgatgttttgggttgaaataaaaatttagaccattatcactctttacttctacaaaaatctacaaa
attatagaaaggctggcttagctaatgctcagaaaatcattttaaagtataatttgaaggaaaaatgataagctcctaacaattcatttcagggaaaaca
gtaaaagtgtaaagtaaacgctataaataggggacattggcagctgcaggaatgggtgccctgcagtgctaatttataaaatgcataatgagtngt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: