XB-CLONE-3546644
Clone Name: IMAGE:7716823
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Gene:
fam3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977878 [+]
atgtaattatgatcaatagcagaagtatgatgctcccctgtgtatcccttctcatcaagaggattgggcaggtagaacagcaacagtgtgttaggtagga
aagctcaccggcatagtcccatgctcttgtggaggttagggcaaggcatatttgctaacctatttccctagcacttctgtgtccctaagctaaggccagt
aatgcctgttaggaagactactcctttgctattatcctggatggagcaaatggctgctctttctgtataattctgtctgaactcaccactactagagggt
gctatgaaatatgctgctatactgtcttttcctcattcacggggccctgtccccgacttaatttggatggatagtggcttactgggccggatctgaaccc
aggctcgatggagctctgcttcaggtagcagatcagcagccaaagaggaaaacacttccttgtgcaagacactatatagtctgccaaggggagtggttaa
cctgcactaaacctatgagggatagtgtttcagctgtaacctgagtacagagggctctgccctataacagtacagatatcagaaagtagggatatgaagc
catagggagcttaaccctatgggtccctacattcacccgggggaagaatccattccgacccggcaatggtaaataacaataagtacactttcaagcaata
aacagtagtgcattgaattagtgcaaaaatacagtacccttacatccgtttctgataccctctcctgaggagtatccggtacattggatagctgtaaaat
gaaaaaagttaaggc
3' EST: CX977877 [+]tctacatgccctatgccccagagtgggtttacgacgaggtggatagacttttagatgagtggctctatggagagttgatccatcgggtaggcctgattcc
aggaggcctcctacatcaaaacaccaccaggaggaaccaggatgcagaatatctggctaacatcgcccgtgagtggtggtttggccgaaccatactcaag
catcgtgtcaggtcctgtggcaagtaccaggaggaccgggatttctcgttgtggatggatcgtcctggaggagagaagaccgacaagccacatccggctt
attatatttcttatgcagccacaaaagccgagtttgaccgtagcctccattgaaaaaccccatgccggttggttcccatacctgatgggattttcccccg
ggtgggaggtgggtctgttttccctaaaaaaggtaagggcaacacccaccatatttgggcacatacccgttggactcccgctagtttgagactaaggatg
tgaccctttttcttcagttacccacagtgtgggcttgttcttttgttcttctgtttggaactctctaccctaaggagagactgtaaagttgacttgtata
tagttgggtgtacaaacaaagactgctgcgccccacctcactacaacttctacttcaacctacctaagggtaggacccattgccttatccacagctacgg
agtgcatgaagggtagtcatgaacactgggttagggtgggaaggcatggacttaataaagttgatgggtgggaatgtgtcacagttaaacttgtgcccac
taacatttctttacagccaagcatgtgcctgggtcacctgaatgaaggcagga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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