XB-CLONE-3546632
Clone Name: IMAGE:7717063
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Gene:
hmgcs1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977887 [+]
cggtcagccatcatgcctggatcccttcctccaaacggtgaaagctcttggcgcaaagatgtgggcatcgttgctctagagatttactttccgtcccagt
atgtcgatcaagtagagctggagaaattcgatggagtcagcgctgggaaatacaccatcggcttggggcagtgtaagatgggcttctgctcggacagaga
agacatcaattctttgtgtctcacagtggtccaaaggttaatggagaggaacaatctgtcttacgactgcattggccgactggaggtgggaacagagaca
atcattgacaagtccaaatctgttaagactgtcctcatgcaactgtttgaagattcgggtaacaccgacgtggaaggcatcgacacaacaaatgcatgtt
acggaggcacggcagctctcttcaatgccgtcaactgggtggaatcaagttcttgggatggacgctatgcgcttgtggttgctggagacattgctgtgta
tgccacaggaagtgccaggccaaccggaggagccggagctgttgccatgcttgtggggccgaatgccactttagtttttgaaagaggactgcgcggaaca
cacatgcaacatgcctatgatttttataagccagatatggaatcggaataccccgtcgtagatggaaagctttcaatacagtgctacctcagcgcattag
atcactgttacactacttatcgcaagaaaatccatgcccagtggcagaaaggtttgttacaaaaatcagttgctccattcatgttttcataaaacattgt
atgcagctgttacatttgtgtgatttacaatttgc
3' EST: CX977886 [+]ttttttttttttctgcccataaggaaaactttatttttgtaaagcatttccattacttacagatttcattttattccttcagcgcagaaggaatcgggtt
aattgagtgaaccattgtctccacatagaacaattcaagtcacgtgacatcaccaattctcgggacacaatactatctcttactgtacatttgtttcatt
agcaaaaaatctgtaactgtacaaaagtcgcagggtgtgaggagaagtattttacaaatgcaggaaaaataacagttaacccctttaagtactgcagcag
ccagcggagccttgtttggcccaaaatccaattacattgtagtgtatgctgcagatgtgactggcgctacagagaggttacctgctaaacccactttaaa
ttttcaatttctaaagacaaaagaattatacacagcaactttaacaaaaaattaaaaaaaaaaaaaaatttacattttatattactttctctacaatttt
gtttttttacaaattagttgggcttcagcccataccattagcaccaacgtgatcttaaaaagctaccgagggatgaaatataaactctgtacatgccaac
agaccaaaaccataaagaatagaggcagccatgataaaaccaagagaggtacgtagtttgtgtccgatggcgtactaaagcatgtgtgtaggacactgct
gcacctcacagcactgatcctgaacctgattggctcacactaatgctgccaatggtagcgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: