XB-CLONE-3546616
Clone Name: IMAGE:7717017
|
Gene:
ctsc
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX977899 [+]
acatacaatataaatttccttataaaaagcataaattgtccctgattcagacataaggcacaggccagagattccctttcacgaggcggcacagggcgat
tattggatgctgtgccgctcaacaaccctgatgtacaaaaatatatccgctaactatttcagtggttatacgagatacgccgagtgatttcccagtgtat
aacatccaatcaaataaatgtgtccctttattgtgggggggcagctgaaccccaatgatcagtcgcttagtaatagtattacacttgagggaaatgcggg
atatataagtgcttttcacgtttacaggcagcggattttggctctttaggagacataaaaaaggtgatttatcgcacaaagaacacaatagaaatagggc
aagattcaggactaaagctaagtgcagcccacgaggtgaccaaaccgtactctgatttaaagggaaaatgtattctattgaagaagtgactttataaaaa
taacagctgccattttgctagtctcttctcagcctatcaccacttgagagagaagaaccaatggcaacagtgccatcttgtgatccacaaatactgctct
ccccacctggttgcaggttatttattcctgctctactagcacataaacagggtacatgttgcctttaaatttagtgcacagagtagggtcctaacaaacc
ccacagttaacaaaaatgaagcaggtctggtcactcatgtgaatagggaaaaacaaaaaccaaaaggtcattggataagacctatggggttatgtaataa
aaggtaccaggagcggaaacccatagncaccaaaaagcagtagcatttaccctgtaaaaaaccaaaacatctcttggttgctatggggtaccgcc
3' EST: CX977898 [+]ctgcaacgaggcctacatgaagctggagctcgttctcggcggccctctctcagtcgcctttgaggtgtatgacgacttcatgcactatcgcagcggcgtc
taccaccacactggcctgcaagataagttcaaccccttccagctgaccaatcacgcggtactcctggtgggatatggcaccgaccaacaaactggggaga
agtactggattgtgaagaatagctggggggagtcctggggggagaaaggatacttcaggatacgcagaggcaccgacgagtgtgccattgaaagtatcgc
tgtatctgcagagcccattattaaactctaacccccatatgtaattaaagtgtgcccaggggcggtaacccatagcaaccaagaagatgtttggtttttt
taacaggtaaatgctacttgctttttggttgctatgggtttccgctcctggtaccttttattacataaccccataggtcttatccaatgaccttttggtt
tttgtttttccctattcacatgagtgaccagacctgcttcatttttgttaactgtggggtttgttaggaccctactctgtgcactaaatttaaaggcaac
atgtaccctgtttatgtgctagtagagcaggaataaataacctgcaaccaggtggggagagcagtatttgtggatcacaagatggcactgttgccattgg
ttcttctctctcaagtggtgataggctgagaagagactagcanaatggcagctgttatttttataaagtcacttcttcaatagaatacattttcccttta
aatcagagtac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: