XB-CLONE-3546523
Clone Name: IMAGE:7716787
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Gene:
tagln
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977961 [+]
ccgagggttttctcattacattacgagtctcattaaacttactgctggattttaatgcaaacatcaaatatttaaacttattattgcatattggtgtaaa
atacacctttaagtgccagtagatgtacagaatggtttcttttttatttagaatgtctaaaaaaaaaaacacatgaaccccattagcagacctagcgggg
atgtattgtgtaattatctgaaaaccaagtttcacattcatactgccctctgattggacatgcaactgtatcacttaacaagcatcaacatcatgtttct
ggttggcttcacagcttgctccaatcacattgccatgaccaggaagcttgatgtgtgctaggaagcctgggtgggaaggagctaacctagcactactcac
tactaccaagacaatgttaattgtaaaggtattttacaacaaatcacaactaataaagtttatttttgtaattctgttggtttccaattgactaatttca
gatttaggacacactagacattttattgcagtgatcaaacgtcccttaatctctgccaactaattcccataaaagtgtatggggaaaaaactgttggatc
aagagtattatctgaaatcctagtgattataatctgatgtctgggttctttggtgatgtgcactttggagtggcattagcagtgggattcaatacttgta
caatctgttacctgtgggttaaggtatgtcagattgttgtagttttcattgatgcagtcacagtgccagcttgctgtattcctaaatcaactattgaact
cttcagttttaggcgtttgacagggggtggtgcagtgatg
3' EST: CX977960 [+]tgcagtgctgtctacttttctactgactggctttgatttcatcttctctgcataatttaataaatgaatttgttttgtaattgcaaaaattgtctcccca
agtgtagcctccttgtcttcactatacaatgtagtcacctctctatagcatattagaaatcacatctcatcactgcaccaccccctgtcaaacgcctaaa
actgaagagttcaatagttgatttaggaatacagcaagctggcactgtgactgcatcaatgaaaactacaacaatctgacataccttaacccacaggtaa
cagattgtacaagtattgaatcccactgctaatgccactccaaagtgcacatcaccaaagaacccagacatcagattataatcactaggatttcagataa
tactcttgatccaacagttttttccccatacacttttatgggaattagttggcagagattaagggacgtttgatcactgcaataaaatgtctagtgtgtc
ctaaatctgaaattagtcaattggaaaccaacagaattacaaaaataaactttattagttgtgatttgttgtaaaatacctttacaattaacattgtctt
ggtagtagtgagtagtgctaggttagctccttcccacccaggcttcctagcacacatcaagcttcctggtcatggcaatgtgattggagcaagctgtgaa
gccaaccagaaacatgatgttgatgcttgttaagtgatacagttgcatgtccaatcagagggcagtatgaatgtgaaacttggttttcagataattacac
aatacatccccgctaggtctgctaatggggttcatgtgtttttttttttagacattctaaataaaaaagaaaccattctgtacatcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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