XB-CLONE-3546490
Clone Name: IMAGE:7717077
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Gene:
swap70
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977984
[+]
caagtctcagctcaaagttctgtcgcacaatctctgcacgttactcaacgtcgcacacgatccggtggccttggaggaacatttcggggataatgacttg
ggtcccgtgtccaaccaaggctacatgccctatctcaacaaattcatcctcgataaggtccaagaaggtttcgacaaggtggaattcaataggatgtgct
ggactctgtgcgcaaggaaacacctccccacgactcccctgtgcatcaacgacgacagcgccttcaaagtatgggccatattcaactacttgtctgaaga
aaagtacccgctgctcattgtgcccgaggagattgagtacttgctgaagaagctgacagaagccatgggaggaggttggcagcaggccctgttcgaggac
tacaaagtggcactggataagaaacaaaatggcctctcggcctgggaactgatcgagctcattggaagcggccagttcagcaaaggcatggaccatgcca
ccgtatccatggcgatcaacgaagtctttaacgagctccttctggacattctcaagcagggatatttgttgaagaaaggccacaagaggaagaactggac
cgagcgctggttcgtgctgaagccaaacatagtaacgtactattgcagcgaggatctgaaggacaaaaagggggaaattattctggatgaaaactgcttg
gtggagtcgttgccagacaaagaggggagaaagtgtctctttgtggtgaaatgcctggagaaaagttacgagatcagtgcatcggataaaaagaagaagc
aagaatggattcaagccatacagacggtggtaaatctgcacaagttccaactcc
3' EST: CX977983
[+]gtactgaaacttgattttatagacggaatttgttacattgaaggaatttatcacaaaacacaattattttagaattcagtttgaataaaaaataacaaaa
aaaacctcagtaataaaattagtcgcaattctccgtggggccgacgctggcggaatgttaaactaattagtggaacctcagtcgtttgggggtgaccaga
cagagccctggtggtcctgattggacgctgggtatctgatgtcacagaccggggcttatttcccacccaatgagccgcggggggtggggcgttagcttga
gatctcattaacacaagttacaatttcacgtaactgacgcgacgcagaaactggaagatgttaacacactaaagccacaccgttagtcctgcaccctaat
ccttcatgccggcaccccggggtgctagtccgtactggggctcctcttctcttgccaagacttctctctctgctccagttccccttgggtgaaggacgcg
ggcccccagtttgtgacaagcccaggattctttgagcctggctgtattattcgcattaacccttcatgttgcagtactttgtctttccaagatctggtct
tgctggcggcctcctctaggttcttggacacttgctggtactgctccagcgccagaatccgctccttctccagctgctccagctgctgcatcgcctcctg
cagggccttctccttctcctcccggatctgctccaactccttcttctctgcctccgtcatcttaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: