XB-CLONE-3546412
Clone Name: IMAGE:7716942
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Gene:
cox20
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978039
[+]
ccgaggttgttccttagggatttcatcagggttgccacacctagcctttatttaaaactactgccaatactgtcactttgtggcctgtagtatttaagca
ttgtgggtatgtacccacttgcacttaacaatctacatgtgttcctcttatgtggcatctgctccagtgtgagaggacatcagtgctgaaggcagaaaac
aggtttccctttaccccacagggcagaccagttggctccagtttagtgccatgaggcaaaagaattatggactgcactgtgtaatacatcctatgtagtg
ctgtacccaattgtcaacgccacagggcatattttatagaaaaaaaatataaaggtggtggtcatttaacagttactgaaaaggtgtggtttattgttac
tgccacaaactcctatattctttaaatgcaggttttggaaaggtgtttagcatatgcattggaatcctgtttttgatgcggaaaatcaggtttttgagtc
agttgtgtttttacgcgtatttggatctatactgctgccttcaaatagtatcctatttttaattccttcttgtaacatcttttgctggatccttactttt
gcattattgtatctgcagtgcatcctgcaaattaaaacaaaaatactgttattgattctgccaaaaaacttttaatactttaaaacttttaacttaattt
taaacaaatggtaagcttagaatagctaagcttgtttgagtcaatgaatgctgttcttgtatatacaccaccaacatctttagtatgtacatccaagtgc
caaatgctaataaacccccagaatagcaggccatactgacaaatgtgtacaaaccaaggtgtgcctggaatg
3' EST: CX978038
[+]aaaatgtattcagaatggaatttcttcatgctgggcagtccatcttcagctttatctgggtttctgttgaattagttcttttcataatgcaggtttttaa
gctgtccaaccatttagcaaacagaaaactgccatttctggctcagagtgcaggaatcagaaatgaaatgtgagcgcatacttacactatttccctagtt
agtggcaatcacaatcaagactacatggcatcaagcagttgtgttccctattgaaattttttctgcttcattcagtgcccattgagcaaactactcattc
caggcacaccttggtttgtacacatttgtcagtatggcctgctattcttggggtttattagcatttggcacttggatgtacatactaaagatgttggtgg
tgtatatacaagaacagcattcattgactcaaacaagcttagctattctaagcttaccatttgtttaaaattaagttaaaagttttaaagtattaaaagt
tttttggcagaatcaataacagtatttttgttttaatttgcaggatgcactgcagatacaataatgcaaaagtaaggatccagcaaaagatgttacaaga
aggaattaaaaataggatactatttgaaggcagcagtatagatccaaatacgcgtaaaaacacaactgactcaaaaacctgattttccgcatcaaaaaca
ggattccaatgcatatgctaaacacctttccaaaacctgcatttaaagaatataggagtttgtggcagtaacaataaaccacaccttttcagtaactgtt
aaatgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: