XB-CLONE-3546395
Clone Name: IMAGE:7716896
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Gene:
prrg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978050 [+]
ctccaggctctttatcttgcttcctgcttgaattagcagaaaacatgggcagtgttttcctgacagaagataaagccaactcagtcttaaaacgatctcc
tagagcaaacagttttttcgaggagattaaacaagggaacattgagcgagaatgcaaagaagaattgtgcacttatgaagaagcaagagaagcctttgaa
aatgatgagaaaactaatgaattctggaaagaatacactcaaggacaacaaggagatctgaatgcaggaaacaactggtacccgttttaccttgcttttc
ctctgatcatcgtgtttttcatcatccttttagtcattttacttgtgtggaaatgcgtattcaaaaagaaggcacgcagacagactgcttatgctcctcg
cgggattcgagaaaatgctacagaagtggcgattgaccccgatggatttggtatttcacctcaacatggcactgttctataccccatggaagttgcattt
gataggggcaggctccacagtggatacgaatatgatgtgcattctgacacactttcagctgggctatcgaactgcgatcctcccccctcttacgaggaag
ccacaggggagcgaggtgtccgagtaaatgaacccccaccaaatcccacagatccgccgcctgagtatgatgaaatagtcacctcagctgcagctaacac
agtacctcacggagctaccagcactaaataacacaaactgaaggattgctatgtatattgttttgtaattctttttattgcagatgaaatattttaagca
caacgttgaagctgcatagcctttgcctttggacaactttcacctcttgacttttccaccctctttacagtaacactcccactttcatgactataactga
attgtgaattctttg
3' EST: CX978049 [+]aaaatgcacactggttaatagtggttatagtgttatgtcagcatgtcagcaccatctaaaaatggtagagttattgcaaagaatcaagggaaagttaccc
agtaaatttgctggtgtaactaagctaagttttctcagcctcaggtaagtatgtatagataaatgtctccttataatacaattccataaagcccagacag
agctaatagttttcttgtgcaaagaattcacaattcagttatagtcatgaaagtgggagtgttactgtaaagaaggtggaaaagtcaagaggtgaaagtt
gtccaaaggcaaaggctatgcagcttcaacgttgtgcttaaaatatttcatctgcaataaaaagaattacaaaacaatatacatagcaatccttcagttt
gtgttatttagtgctggtagctccgtgaggtactgtgttagctgcagctgaggtgactatttcatcatactcaggcggcggatctgtgggatttggtggg
ggttcatttactcggacacctcgctcccctgtggcttcctcgtaagaggggggaggatcgcagttcgatagcccagctgaaagtgtgtcagaatgcacat
catattcgtatccactgtggagcctgcccctatcaaatgcaacttccatggggtatagaacagtgccatgttgaggtgaaataccaaatccatcggggtc
aatcgccacttctgtagcattttctcgaatcccgcgaggagcataagcagtctgtctgcgtgccttctttttgaatacgcatttccacacaagtaaaatg
actaanaggatgatgaanaacacgatgatcagaggaaaagcaaggtaaaacgggtaccagtttgttccctgcatcagatctcccttgtgtccttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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