XB-CLONE-3546383
Clone Name: IMAGE:7716802
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Gene:
dnajb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978058 [+]
ctaaaagacaagtctcatccagtgttcaaaagagaaggatccgatgtggtttatacatccaaaatcagcctgcgagaggctctatgtggctgttcagtaa
acatcccaaccgtggataaccgcacaatcccgcttacatttactgacatcatccgtccaggaaccaagaggcgaataacgaatgaaggacttccccttcc
taaatctcccgatcagcgcggcgacttaattgtagagttcgacattcggtttccagagaggctgacggccagctcccgcgaagtgttggaaagggttctt
cctgcataaggaccaagccttccctaaggcaccgtgaccaaagcagaactggttcatttaactgtttcagctccagggggcctgggaatggggaaaaatg
atggttaggggtgccacatcctaattttgatcacatcttcatttattccctgattatatttctattccatgaatctaattcagcactttgtaatattaat
ggacgattaatgggaagcgttttggcagtggcagtgctggtagtttgagttaagccacaattgaaatactttttgggtccaggaatttattcagtatact
gtcactccgaaaacccctaatttctggtgctgttgagttgggcgactgtagttgtcttttgtctagtggcgagatattgtgcctccgtgctgaatgccat
tanactggaagcagccactgcatncagtgtcaatgacagatcgaatactggtacttatgtgggaaccaccctctgttgcctctttctgccctgtaaatgg
gcagcttaggggcaagtcgggcagctttttttattgctgtctgcactcactaaagctgcccatacatgc
3' EST: CX978057 [+]ccagtgaatcaaaatagtgttttatttacgaccaggaacattagaagcaattacctgggtttagagatgtgtcacagttatacccttagttagaacagtg
atccccaaccagtggctcaggggcaacatattgctctccaaccccttgggtgttgctccctgtggccttaaagcaggtgcttattttaaaattcttggct
tggaggcaagttttggttgcataaaaacctgatgtactgccagagtctcctgtaggttgccagtccatataggggctaccaaatagccaattacagtcat
tatttggcaccccaggaacatgttttatgctcgtgttgctccccaagtctttttacatttgaatgtggctcacgggtgaaaaaggttggggaaccctgcc
ttagaaccttagtataactgccgagtatcacttgccctttgcttgtcaatgtatcccctattagcgacttgtcgcttaccagctttataagccaatatca
cctggattcggttttttttttatatcaatgccacaatggcatcttccagaaaaactatttatatacaaaaacaaaaaaactttacaaaacatagtacaga
tatgcataacatataaatacacggaatcagttccatacccaccccgacaatggagacaggcttccataagagcaaggctgcaaccagcaatatataggcc
atgcaaccgatatcctaagctccacccaaaaattccattgtggga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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