XB-CLONE-3546103
Clone Name: IMAGE:7716782
|
Gene:
actg1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX978252 [+]
cttgccccatgctatcctgcgtctggacttggctggtcgtgacctgacagactacctcatgaaaatcctgaccgaaagaggctacagcttcaccaccaca
gctgagagagaaattgtgcgtgacatcaaggagaaactctgctatgttgctcttgactttgaacaagagatggcaactgctgcatcctcctcatccttgg
aaaagagctacgaactgcctgatggacaagtaattacaattggaaatgagaggtttagatgcccagaggcactcttccagccttccttcctgggtatgga
atcatgtggcatccatgaaactacattcaactcaatcatgaagtgtgatgttgacatccgtaaggacctgtatgccaacactgtcctgtccggaggtacc
acaatgtatcctggtattgctgacagaatgcagaaggaaatcactgccttggcccccagcaccatgaaaattaagatcattgccccacctgaacgcaaat
actctgtctggattggtggctctatcctggcctccctctccactttccagcagatgtggatcagcaagcaggagtatgatgaatctggcccatccattgt
ccaccgcaaatgcttctaaatggactgctagcagatgcgttgcatttgctgcgtgagttcttcaaagtattaatttgccttggcaaatgtgtacgcctca
tgctagcctcttgaaactggaaaaagggccaacttccaaatgaacttatcctagg
3' EST: CX978251 [+]cttggaagttcaattctcatctggatgacatacaaattttggttttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaactt
ggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggtt
cttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtat
taagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaa
gtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaacca
ggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatccccca
acagtgactatgtgtgtcttcaattcctaggataagttcatttggaagttggccctttttccagtttcaagaggctagcatgaggcgtacacatttgcca
aggcaaattaatactttgaagaactcacgcagcanatgcaacgcatctgctagcagtccatttagaagcatttgcggtggacaatggatgggccagattc
atcatactcctgcttgctgatccacatctgctggaaagtggagagggagccagg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: