XB-CLONE-3545993
Clone Name: IMAGE:7717032
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Gene:
hnrnpl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978332 [+]
aagagacagaagactgacgcatctgaggacaacgtggcggcttcagcggaagggtacgatgaccctcacaagaccgcggcctccccggtggtccatgtga
ggggacttattgatggggttgtggaagcggaccttgcagaggctttgcaggagtttggaacgatcagttatgtcgtggttatgcctaagaaaagacaggc
tctggttgaatttgaggatattcttggtgcatgcaacgctgtaaactacgcagcagacaaccaaatctatgttgcagggcatcctgcatttgtaaattat
tcaactagccaaaagatctcccgtcccacagatacaggcgatgattcccggggggtcaataatgttctgctctttaccattctgaatccaatctattcca
taactacggatgtgctgtataccatctgtaatccttgtggacctgttcagcggatagttattttcaggaaaaatggagtccaggccatggtggaatttga
ctctatgcaaagtgctcagcgtgccaaagcttctctcaatggggctgacatttactctggatgttgtacactaaagattgaatatgctaagccttctcga
ctgaatgtcttcaaaaatgaccaggatacatgggattacacaaatcctgccctgagtggacaaggtaattcacttggttctgttctctgctttttacaca
tttcctatcagttagtctgataaattgcacaagtgtcacttcacgctctgtaatgccatctgcttgtctaatagtcttgtttatatattcaacaaatagc
cattgttttggcttaaactgctttaa
3' EST: CX978331 [+]tttttttgttctaaatgttttattgatatgtgcaagcacatcatcatagcaaaaaaaaaaaaaaaataaaataggaagtcacaacaatatttcagaacat
aaaaactagctccacaaggaaccaaactaatactaaacatcaaaatcagggctaggaaatcagacaggtcttcttaggaggcatgctgagcagtggagaa
gcagagttttagagtgtaggggtaaggaccatttggatttttcatctggtagtgattcatgagaccgagagtctccagtgcttcgctcttagaatcccac
tccagcagtccggaagagcttcgctcactctttccagaaaagattttgattgaagctggcttcttaacacccagttcatcacacatctctataaagttct
cctctgtcacctcaggaggagcattaaagaaatgaagcacactgctcggctgttggattctgttcttggcagcttgctctggggatgtaaatctgttgtt
acgggacccactgaagactttaaaactgcaggagccatcttccaagccataggactgcccaggcacaatagactgttgctttgaaacacagacacttagt
ttttgcccaaacatgaagttgttgtttaagtgtgtaatggcacggtctacagcatatccatcagccatctccaccatagctgctccaggtttactcttca
tgaattttaccttctccacattaccatacaggcagaagacattaaagactcgatcacagttcatctttgaggggtctaaaccatataccatcagtactgg
actgtctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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