XB-CLONE-3545953
Clone Name: IMAGE:7717275
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Gene:
ppp4c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978360 [+]
caaaaatgtaaaattagaagaaaaaaaaaataaaaataaaagggaaaaaggataaaatctttgaccagaaagagtgcggaacaagagactggggccatag
acgaaaggaagggagttataatggaatatcggctgttccagggaaggaatcggaggtttggcattgtaggtggcaatacacgagctgaagctttagatgc
ctccttgccaaagcaatctctcagctagccgcttacaaagggttaaacaaaatggctactcacgttcccggcagcccagaatggcttcgctgagctacag
gggccagctacggtgaacactttggctcaaaagttgccaatcggcaccaaaaggggctcaaatcttagatctgccgcagcttctaaatagagaaggggcg
gcgggttccatcatcatcatcatcatcatgtctctcctggtcccccccagctgggattgggcgcccgggccccgcttgcactaaggcactccgtacgtga
gtgtaagctcagcatcttagtacaggggacgtgaggctccgagtggaagagacattataaggaatgactgcggatctgggggggggggggggttataaaa
tcaaggaagaagctgtgaattctagaagtattttggtttccttgcataatgctgcccccccctctctccctactagaaagcatcacactgaccccccctg
aaagtcctgcagagcattacatttacctagtttc
3' EST: CX978359 [+]gggcgagaggttgaggagggaccatgactgaaatcagtgacctcgaccggcagatcgagcagctgaggcgctgtgagctcatcaaggagagcgaagtgaa
agctctttgtgccaaagcgcgagagattctggtagaagagagtaacgtgcaacgagtcgattcgcctgttacggtctgtggggatatccatgggcagttc
tacgatctcaaggagttgttcagggtgggcggcgacgtccccgagacaaattaccttttcatgggtgactttgtggatcgtgggttttacagcgttgaaa
cattcctccttctcttagcactaaaggttcgttacccagatcggatcaccctgatacgcggcaaccacgagagccggcaaatcacacaagtgtacggttt
ctacgacgagtgtctgcgcaaatacggctcggtaacggtgtggcgctactgcacagagatctttgactacctcagtctgtctgctatcatagatggcaag
atcttttgtgtacatggtgggctgtcaccctccattcagacattggatcagattagaactattgaccgcaagcaagaagtgcctcatgatggacccatgt
gtgacctgctctggagtgacccagaagatactaccggatggggggtgagccccaggggagctggatacctttttggcagtgacgtagtggcacaattcaa
cgcagccaacaacattgatatgatttgccgggctcaccagcttgtcatggaaggatacaagtggcacttcaacgagactgtcttaactgtgtggtcggca
ccaaactactgctacaggtgcgggaatgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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