XB-CLONE-3545897
Clone Name: IMAGE:7717233
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Gene:
gadd45g
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978398 [+]
gccgagggtggcttacccggaaaatcatgactctggaagaagttcacggacaagaaaccgttgttgaaagcgctgacaggatgcaaactgctggcaaagc
cctgcatgaactattggtctctgcccagagagaggagtgtctgaccgttggagtctatgagtctgccaaagtaatgaatgtagatccagatagtgtcact
ttctgcatcttggctgcagacgagtacgacgagggtgatatagcccttcagatccacttcactctgattcaagctttctgctgtgaaaatgacatcaata
tcgtgaggctgaatgacacagaaaaagtagctcaaattctaggcttaacagatgagtctgcagagcccaaggacctgcactgcatccttattacgaatcc
aaatgaggatgcctggaaagatccagccctggagaaactgggtatattctgtgatgaaagccgtaaagtctacgactgggttcccagtatcaccctgcca
gagtgaacatcttttctctacattagattgtaaaatctctgcattattatcatggtgttgtgcacctggatggaccagcagcattccgaagtctgtggat
tacgctggttgaggtcagattaagtcgccaagactggcaaagatgttggctcttgtggagcagaagagtaaggaggaaaacttaacatggactgatctgt
aaaaggaagggttggtgccagtttgaagcctcccaaagcactggggggggggcagtcatgccatggagctggaggagttttgcatgtgctaaaagcaaaa
atggaagctt
3' EST: CX978397 [+]gcgagaggctttaaatgccaagtttcatcaagaaaagcacttcaatttgcaacagtgtcaaaatgttgaaaatacagaaagtctttttttttttttacta
tgtattctgggtagtagactgtagtagtagactgtaataaccaataacaagctgcaatgaacagaattcagtctcagtttgcaatccagagctatatagt
cccagactcttccaatgtgtatataaagttcatgtttcatatctcagcaaactccaaaagcagatacagaacaggttctttcctttaaccagctgcttaa
tttgcctcatatccatcttaaaagcttccatttttgcttttagcacatgcaaaactcctccagctccatggcatgactgccccccccccagtgctttggg
aggcttcaaactggcaccaacccttccttttacagatcagtccatgttaagttttcctccttactcttctgctccacaagagccaacatctttgccagtc
ttggcgacttaatctgacctcaaccagcgtaatccacagacttcggaatgctgctggtccatccaggtgcacaacaccatgataataatgcagagatttt
acaatctaatgtagagaaaagatgttcactctggcagggtgatactgggaacccagtcgtagactttacggctttcatcacagaatatacccagtttctc
cagggctggatctttccaggcatcctcatttggattcgtaataaggatgcagtgcaggtccttgggctctgcagactcatctgttaagcctagaatttga
gctactttttctgtgtcattcagcctcacgatattgatgtcattttcacagcagaaagcttgaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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