XB-CLONE-3545880
Clone Name: IMAGE:7717187
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Gene:
ddit4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978412 [+]
cccccatcttggaggaagagccccaatcccagttttggggtgaggactgcgcagatgtgtcgcaaaggtgctccagtttgcccagttccgactgcgaatc
tctcaccagtagcaactcttattctgaatgtggtaagtctgcaaataattatttattgcatttagattaaatgagatttaaaacatgttttatataatac
tttctgcaaaggcttgtcaccctggcctataggggcactgttaatcaactactggattccttcactaagcagtatggaaacccctatgctctgtaggaat
taaaaatatgcgtagtgggtagtttatttagatatataatattataacacttatatgtgtttttttgtttttttttgtaattaatgcacggacatcctgc
agatcagtatataaatgcatagcacatcccttcccttagaattattaaagctttataactgcagctgttttccctaaatgtgcaactaatcacaattttt
ttttttttttctattcagatctagatgcttgggatgaaatctcattaccagactcagagcttctgaatgatcctgaaggagagcagctctgccccagtct
tctaaagcttataaaacgatgcctgaccaaagctaaaatcaactccctgaggtgttctaggcttcttattcctgatgaactcttgtgtaatctgggtcaa
gaacttctacacttggcgtacagtgaaccatgtggacttaggggagcacttattgacttgtgtgttgagcatggcaaggactgccacagtgtggcccaga
tcactgttgatcaagctgttgtacctacttttccagcttactgtgctgctga
3' EST: CX978411 [+]ttttttacatttcattgtttttaatagaaaagtaaaaaaggcacattgtgtgtagttttacttgaacatcaagtgtatcactgtaaaccataataaagca
gcatattagagtacataggacacattttttttaataaaagggtagtgctttagaacattctgctgcctgaatctgaagaaaaaaaaaaaaatttataaca
ccacataacatagctgccatagcatcagtagtcaggcgatgtgaacaatataacacttggtccccagagggaaattatactttttggttttgcaaaaaaa
ctttaccctcttcaagaggtcgggcatattcaccctttagtgcagaccagctgctgatacagtacagaaggaaaaaaaaaaaaaaaaaagccaccagtgc
actttatacagttatcaatgatttacgtgcataaattcactacaaaatttattcttcaaaaagcagagtgaaatgtattcctttaaaatcccgccatctc
ccaaatggatcagaaccatttcactactgtggcaatgagcatttaacaaggcatcgctctttactctgtagtgaaggggttaaccagagacagacagtgt
cctaacaccatctaaaagcattttttttttttgtaaaaaaaaaaaaaattttaacataaaacattttagtaaacttttaaaatacttttaagccttcatg
attgcagtaccaagtaacactagcgccccccacacttctgccactgaaatatcagcattcctctataataagttcttcagaactatacagtttcttttta
agaaccttgaatccagggctaagtttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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