XB-CLONE-3545867
Clone Name: IMAGE:7717427
|
Gene:
atg2b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC160599 [+]
gaacctcatccagtatcttgctagctatggagacctccacccaccttcccggactgaaacctcacctggtgcacacaagaaaaacaaaacgaccgatcca
gtaactcaagcaaatatacaaggtccagtcctggctgaggcagagcagcagatactgagagatttgatgagtgatgccatggaagatacagatcttaacc
atattgtttgcccgtcaaaaccacatctgaatggaatttctgaagggagatgtgagacccaggaacccccatgctcagatctctttctcttccctgatga
aagcggcaatgtgtctgaagaatctagtcccatgtttcctcccaccactccccatcttcttagtgaagccatgttggattttccatcagaaagtgatgac
ttctgcatcctgtatgctccaaaaaattctgtttcggaaaaggatgaagaaccatctgtgaagaagcttgttgatgataacattttaataaaagaaaatc
actttaacctgcctctgcagaaaatggacacaagtaaagcaccattgcactttcctgtgccagttgtacgttatgtggtaaaggaagtgtcacttgtttg
gcatctttatggtggcaaagattttggcagtgctttaccaccactatcacctgctaaacattatatcagtcctcatagctctccatcaaatacccccact
aggcgtgggaacagcttagtgcgtggtggacgagggagaaatcaagatcttttgatggaaatacaatttagtaaggttaaatttcagcatgaagtatacc
ctcagggagcatccacggtagaatctccatcgatggagcaaccagtatcacgtcaggtctttattgtgcaggatttagagattagggatcgcctggcttc
atctcaaatgaacaaattccttcatttatacagcagtaaagaaatgccaaggaaagcacattccaacatgttaactatcaaagccttacatgtgcgtcct
gagactggtcagtccccacaggaatgttgcctgagagtgaccttaatgccactccgacttaatattgaccaggatgccttgtttttcttgaaggattttt
ttacaaatctttctacagaggttgaaatattggtcacgccagatccagaagtaaaaagatacccagcttctgatgttacgtgcagtttgccaaagcacct
ctattctcaagagcgaaaccctgttatttcatttattgggcaaagtgagccagagaaaaatggcagcatcaattctgaacatattgcacaaagtgcctca
gaagaggcattcagtgaccagcctgtctttttccgggaatttcgatttacttctgaagttccaataaggctggactaccatggaaaacatctctcaatgg
accagggaacactaactggaattcttattggactagctcagttgaacaattctgaactcaagctgaagaggctttgctaccgacatggtttattaggttt
ggataaactcttttcctatgctgttaatgaatggcttacagatattaagaaaacccagcttcctggaatattgggaggggttgggccaatgcattctcta
gtgcagcttgttcagggactgaaggacttggtgtggctgcctattgaacagtataggaaagatggtcgcatcgtccgaggtttccagaaaggagctgcat
cttttggaacgtctactgccatggcagctctggaactgactaaccgaatagttcaaactattcaggctgctgcagaaacagcgtatgatatggtgtcccc
tggaccaagccctgtcgagcttaagagagcaaaacgattttcccatcatcgattggtgcatcagcctgtggatctaagggaaggggtggctaaagcatat
actgttgttaaagagggcattacagatacggctcagaccatctatgagacagcagcccgtgagcatgaaaacagaggtgtgacaggagctgttggaggag
ttttaagacagattcctcctgcagttgttaaacctctaatagttgcgactgaagccacttcaaatgttttgggtggtatgaggaatcaaatcaggcctga
tgttcgagaggaagagtctcagaaatggaggctggaagagggttagtttcatgctttgtggctgtgcgtttgcctgacaaagaagtaatggaagaccccc
tccccctcaaggttacaatttgtgatgtttcagcaaatttggatcaggaatctggttacttttttgatgacaaaaagggaaaaaatagacactacatagc
ttttgaaaattattatttacaccgacatctgtgactttgccccccactcattcagttgttcatctgtatgcatggttctctgaacaggttctaaactggg
ggtcatttcagctggatggtttccattcttttggcatgtgtgctatttgcaacataccctcagcaattaatagctagtaatgaaccttgcgtccgaacaa
actttccaatctggcagacgacaaagtaatataagtgtgtttggatgtttgcttgatggactccttggattctcctctgcaatgcactctggtttaatgt
tgtttaatgagttgttctgtgttgctatttgactctggaaagcctggatacagcagaaatatgtaaattgtagtccttattctgtaaattgtacttgtta
atagtgtgaataaagttgacgtcctggtgaaaaaaaaaaaaaaaaaa
5' EST: CX978422 [+]aacctcatccagtatcttgctagctatggagacctccacccaccttcccggactgaaacctcacctggtgcacacaagaaaaacaaaacgaccgatccag
taactcaagcaaatatacaaggtccagtcctggctgaggcagagcagcagatactgagagatttgatgagtgatgccatggaagatacagatcttaacca
tattgtttgcccgtcaaaaccacatctgaatggaatttctgaagggagatgtgagacccaggaacccccatgctcagatctctttctcttccctgatgaa
agcggcaatgtgtctgaagaatctagtcccatgtttcctcccaccactccccatcttcttagtgaagccatgttggattttccatcagaaagtgatgact
tctgcatcctgtatgctccaaaaaattctgtttcggaaaaggatgaagaaccatctgtgaagaagcttgttgatgataacattttaataaaagaaaatca
ctttaacctgcctctgcagaaaatggacacaagtaaagcaccattgcactttcctgtgccagttgtacgttatgtggtaaaggaagtgtcacttgtttgg
catctttatggtggcaaagattttggcagtgctttaccaccactatcacctgctaaacattatatcagtcctcatagctctccatcanatacccccacta
ggcgtgggaacagcttagtgcgtggtggacgagggagaaatcaagatcttttgatggaaatacaatttagta
3' EST: CX978421 [+]cccaggacgtcaactttattcacactattaacaagtacaatttacagaataaggactacaatttacatatttctgctgtatccaggctttccagagtcaa
atagcaacacagaacaactcattaaacaacattaaaccagagtgcattgcagaggagaatccaaggagtccatcaagcaaacatccaaacacacttatat
tactttgtcgtctgccagattggaaagtttgttcggacgcaaggttcattactagctattaattgctgagggtatgttgcaaatagcacacatgccaaaa
gaatggaaaccatccagctgaaatgacccccagtttagaacctgttcagagaaccatgcatacagatgaacaactgaatgagtggggggcaaagtcacag
atgtcggtgtaaataataattttcaaaagctatgtagtgtctattttttccctttttgtcatcaaaaaagtaaccagattcctgatccaaatttgctgaa
acatcacaaattgtaaccttgagggggagggggtcttccattacttctttgtcaggcaaacgcacagccacaaagcatgaaactaaccctcttccagcct
ccatttctgagactcttcctctcgaacatcaggcctgatttgattcctcataccacccaaaacatttgaagtggcttcagtcgcaactattagaggttta
acaactgcaggaggaatctgtcttanaactcctccaacagctcctgtcacacctctgttttcatgctcacgggctgctgtctcatagatggtctgagccg
tatctgtaatgccctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: