XB-CLONE-3545862
Clone Name: IMAGE:7717141
|
Gene:
nr2f6
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX978425 [+]
gtaactgcagcctttggggcgggctgaggagcagccgatcctgctgctagcggcctttccagactctttttttattttgttaaattggtgctggtatggc
catggtgtctgggggctggggagaccccaacggagacactaatggtgtggggaagggataccccaggaattctgaggaagaagaggcctcgcctcaggga
ggaatgagtgaccccgagcagggtgatgaagaacgcccagggatccaagtggactgtgtggtgtgtggagataaatctagcggaaagcattatggggttt
tcacctgtgagggatgcaaaagtttctttaaaaggagtgtccgcaggaatctcagttacacttgtaggtcaaaccgagattgtcagattgatcagcatca
tcgaaaccagtgtcagtactgccgactgaagaagtgcttccgagttggcatgcggaaagaagctgtccagcgaggtagaatcccacctgcccactctagt
gccagtcccacttctgctccgggagcaggcgagtacttcaatggacagcccgtctctgaactgatctcccagctcctgagagctgagccatatccagctt
cccgctatgggtcccagtacgctcaacagggtagtgtgatgggcattgataatatctgtgagttggcggcacggcttctctttagcactgttgaatggtc
aaggaacatcccctatttcccagaacttgctatggctgatcaggtctcattgcttcgactgagctggagtggactc
3' EST: CX978424 [+]gaccataaaatatgtttaatatcaacacttctgcatctctccattgtgtaaaattcctggaagctgaattcagaagaacaaaacagttgacttcagttga
ctgggaaattagtgttcgatcaagaggtagacaaggtgtggactaagctgtactgtcagaggcatacaaattcttatgggtttatacagagttcctgggg
tgatctggaactcttgctgattaaagcatattttagggcacctttagaacatctaaaatatcctacagtactcagcaaaattaagggttactatttatct
atacaggaaatgagaaaatgtaggcggtattctgccagaaagtttttttttagccctgatgtaacccaagcaaattatcagcagagagaaaattcatggg
tctgtagacatctcatcataaaatttggaagtgcccttgtgacttgcaaaagaggttgagcctgatctccagaaataggcaaaggcctaccaccagacta
agtatccatggtgtggcccaagtcaatattattgcactcaagcatctggtggtgctattgaccagatgagtatggccaattaaaactactcccagacaga
agcatgtccctaataagtgtttctataggggtcttgccgacaagcctcatgaagaagagctgggatatgagtgatgcaggaacagctcgaagtgcaggca
gtcgtaagaggagacgcccaaagcgctggggctgagagggatactgggctcggacatattctgtgagggctaactgggctttttcttgtaagcttccacg
tgagctggatctgtgaggccacaggcatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: