XB-CLONE-3545741
Clone Name: IMAGE:7717439
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Gene:
ccl5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978505 [+]
tataatggagccgagtctgcaccagttggttcagatgtcgtctcctgctgttttgattacataaagaagccaatcccacccaaacacgtggctgattact
tctacaccagtagccgctgctcccgatttgcagttgtccttgtcacccgaaagaatcgaaagatatgtgcaaatccagaagatgaatgggtcaataacat
cattaatgtcctggaggtgcctgaaacacaatcataagagtgcagtttgctcaatattaccatcctctgtataatacaatgcaaatttataaccagcagt
cctttatcttaaactgtcctgctttttattgataattgcattaagaaaataaattttgttatgtaggagctttttacctgttgtagtcagtacccctctc
ctcatgcttcctgttgtaaggtgaactgctgggttcaatgataccgtgttgcagcaaagtattaatttcatgtgccacacctagttctgactgggattta
agagggtattgcggtactctaggactgtcacaccctggactgaggcgaatttggacctctctgacagatttaactttgcgttaacttttgtgtagcacag
actgagtcatttatatcagcaaagcgatcagggggggttggaatttgaagacatggcagcaagaaatacagtagaattaaaatccaatataacattagct
ttagcaagaaggttgcgtcaaagaagattaactggccaattaagtgcatagacaacggaatgggaaaagtacaatctccaacagttacagaagtttcagc
agtgagatgggtaatctgaggtttaccgtcaaaacat
3' EST: CX978504 [+]ccctctttaatcacttcactgacacttgccgcaactaatctgtgtccccatagcatgtccagaactacagagccgtttggccccccacctttagctgtgc
cccttctgttcccagcagccatattggtaatcagcaaacagcacaggcacactggcacccaaactgggatattggggtacaaggttgaactggcccaaga
gggcactggataaagacccggtgggccctagtcctgttggacctcttttcaccaggcacgttggtgtgtggattgctgctgaccagtaatgagtaggagc
tagagaagacagtatttagacccttaaatctagttacagaagtggaattacacataaatatagaaaacattagaaagcccagcttgtcctgaaaaaaagg
atgtatagtacaggcaaactaaaattacccctcaggaaatgccccaaagtgaaatgacaaatggcatatgaaatgcaaaatcctgctggtaaaatcatta
tcccaaggctcctgtagatatgggactgggttggatggcactgacagatttactaagagcaaaatgacaaaatctgaaaactgtctgtttaaaagacaaa
ttcacaaaagtggagatagaggtttgtgaataaggtggaaaatcagacaaatctatctccatttttatttgtctcaatttatttgtcttttgtgaattcc
cccaatatgttgttgcggattcctatgtccacagaggaacccgtagctaatatttcagtagctggttttctttgccttttctgcttgattccggcactac
tatatctgtcctctctgctctccctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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