XB-CLONE-3545720
Clone Name: IMAGE:7717441
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Gene:
cela3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978519 [+]
ccatcgattcgttacgctccatctgcccgagtggtgaatggtgaaagcgccaaaccctacagttggccttggcaggtctctctgcaggtcctgataaatg
gggtatttatacacaattgtgggggaaccctcatcgctgacagatggattctgactgccgcccactgtatcaacttctccaggaccaatcgggccgtggt
tggagattatgacttagccaatgaggaaggagcagaggagatcttccttattccatctgaggacatgtttgtgcaccaatcatggaacaataattgtatc
ccttgtgggaatgacattgcccttatccgattgccccgccctgtgcagataagtgataaggttcaactgagctgcctcccccccgccggggaactgctcc
ccaacaatttctcctgctatgccagtggctgggggcgactttacactggtgggccctatcccgatatcctgcagcaggcgctgctcccggtggtggatta
tgatcactgcacccagagagactggtggggcgccacagtgaaacgcagcatggtgtgcgccggaggggacattcgctctgtctgcaatggtgactctggg
ggacccctgaactgccagggggccgatggccggtggtacgtacatggggtgaccagctttgggtctggctacgggtgcaacaccctcaagaagcccagtg
tgttcactcgcgtgtcggcctttaactcttggatccagcanaccatcagtgagaattccgtaagtggattccagtagatccttcccggggaatctggccg
ggaatctgggcggagtcatctcttctattggtcttgtactgaatctccggaacacagat
3' EST: CX978518 [+]gaattgctgaggatttattggatctgtgttccggagaatcagtacaaggaccaatagaagagatgactccgcccagattcccggccaggattcccgggaa
ggatctactggaatccacttacggaattctcactgatggtttgctggatccaagagttaaaggccgacacgcgagtgaacacactgggcttcttgagggt
gttgcacccgtagccagacccaaagctggtcaccccatgtacgtaccaccggccatcggccccctggcagttcaggggtcccccagagtcaccattgcag
acagagcgaatgtcccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtctctctgggtgcagtgatcataatccaccaccgggagca
gcgcctgctgcaggatatcgggatagggcccaccagtgtaaagtcgcccccagccactggcatagcaggagaaattgttggggagcagttccccggcggg
ggggaggcagctcagttgaaccttatcacttatctgcacagggcggggcaatcggataagggcaatgtcattcccacaagggatacaattattgttccat
gattggtgcacaaacatgtcctcagatggaataaggaagatctcctctgctccttcctcattggctaagtcataatctccaaccacggcccgattggtcc
tggagaagttgatacagtgggcggcagtcagaatccatctgtcagcgatgagggttcccccacaattgtgtataaataccccatttatcangacctgcag
agagacctgccaagggcaactgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: