XB-CLONE-3545688
Clone Name: IMAGE:7717253
|
Gene:
csrp1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX978540 [+]
aagagcacatgaaaatatgcacattattaggcatttattattatatgtacattaataaatgaaataagacattcgggaggaatgaagaagcactttatta
taaagcataatgaattctcgatacagaattggtcatgtgatcacattgctccaaacccgagcgagtttattggtttacaggtcggtttcactgaaactaa
cagattttgggaaggatcacaccgagaggcggcaccgtgtggcccagtaatggggaatagaaaacccgttgcagactgataataacaacaagagaaagag
cgtaagggcatttacagcggcggctgatttcccaggaatgacagcggaatccggcctggtacctataggaaaatagtgcattaatggagagaacaagtgg
cgttagtgctgaatctgtaaaaaagaagaggaatgaagagattagtgcagcccggcagcggaacgcgttttgctgcctctttcctacctgcaaataaaat
cacgttaagggcaggagctcctaaagggaaacaggttaggcttgtggggagagaagctggaaagaaagaggttaaacagcattggatctgacctcctaga
aagagaaagccattaagaaaaaggctatggcagccatggttccccactgctgctccccttcattaagaagcaccctatcactaatgngaacccggtgctg
ctctaggctctccctacggatccccgtgccgctgcccaggcactcacatatgtcccactagccattcacgcttagtggttagactggatgagtggtggga
ctggaccaggccgtacnatgggcagtcagtacactagccattactagtagccctgtgtctagtgacagaaatgtgccataaagt
3' EST: CX978539 [+]gctttaagataaattgccatttgagcaaccttacaagtattgagttaatgtgtatttttaattttcagcaaagtgaggagctatgtgctgtgctaccagg
cccagagcatggcgtttcagggcatggacataggggtatgagcataggcaggggcggggccagcagcacttggtaggtacatactatgtgtagggggtct
ctgcctgtgagacataagagtgctcttttgggtaccattatgggcagccaggatccttcagggctccagcacttatttatttccattagattcctagttt
tgcacattgcagattctatgcactgatctttggggtgcctatagggggcgctcattcacagtttaaatatagtcacactgatttaccattaaatcctatg
gatttgtgtttttatggcattggccttgctccgtgggaaggttcttactggattccatgttgggatcttattgttatattgtcacattgccggggaggat
ggtggtatagctgtcactctgcccagctgtgcgcttcctaacggcactttatggcacatttctgtcactagacacagggctactagtaatggctagtgta
ctgactgccccatgtacggcctggtccagtcccaccactcatccagtctaaccactaagcgtgaatggctagtgngacatatgtgagtgcctgggcagcg
gcacggggatccgtagggagagcctagagcagcaccgggttcccattagtgatagggtgcttcttaatgaaggggagcagcagtggggaaccatggctgc
catagcctttttccttaatggctttctctttctaggaggtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: