XB-CLONE-3545685
Clone Name: IMAGE:7717301
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Gene:
mrtfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978542 [+]
ccatgatgtgatttatttttttttaatttttttggtattttacttccttttatgaatttactcattttttttgcctatgtctcacaacattactgagaaa
agacagatattcatccgcattttacattcatttcacagatgtgagcaaggattccaggtacccagcattcatgatgggaaaacataaaaaagaaaggaaa
gagaaaaataaattgttcaccaaaccagaggtgggtcgatagtgaaagaacccaaaccgtttgcagaacacaccaaggatataaactgtcatgagttcgg
caagggttccagttgggtgaacagaagtgccatagattgaacacgatatgagcaactataaggggaaaaagtggggctttgcgtttacaccaagagtcat
ggctgaacttaatctcctcagcagtttcagattcctgggaagggggacctagaaattatacagcagcactaaccttagtctttggatatcagatgctgaa
ttcccagccatgtatttctacccagtagttacaaatggcaagataacatcactaccttccagacaggcatatcgaccaggggtgacttacagcactggac
agtttcttacaagtgcttgggatgagctttgacacagaaagcaatataaagggcaaggtatctgaagctaaggaagcttgtggcacatttgaacagtgca
tcaaggatgaacaactggttagagacaatgcatagttcttattgacttgcttacccatcttacatgtgtctttaatgaaggtagtcaggggccttctcaa
gggtagcaactaaggattccctacaatttggcaccctgaactgagcattttctttcagtttccgtaagtaccactc
3' EST: CX978541 [+]gaggtcgagacaagaccttatgctacgtgagaaggacaagcagattgaagagctaacacggagactgaaacaaaaacaggagttagtagaacggctcagg
cagcagcttgagcaggagaaacggacacagcaacatcacagtgccgacgaccagcaggctttaattcttgcagttaaacaagagcctgtgtccctaacca
ttgattccattaataacaaaaaggagacctctgaggcgagcaataatgtgaagaaggaacttaaccgggttatcgcttgccagcaagaaacacagctttt
aataggttctatgtccggtggcattgaagggaaagtagacggctctggaggaacaaaattagtctttacactggccagccctagcagccagcttcctgaa
gaaaacaggcaaatagttttacagagagttcctaccccgccatcatctctccaccctaccaactcgctgccaaagcaagaggtgctccttaaccactgtg
ccctccagaaccagaagccggccctgcagcttatgccagggactgtcttgtcattgtcaccttctaatctgcagcccattttaaacatgaacggattcca
gaaacggccagcggacacttccaactcctctatacagaaacagaagccggtgcataatgaatctccagcaacaccgcccccacagccagagcctgagccg
ccaccacacagtatttttttaactgcttcttcacctccatggtcaaaagatccccctggatatgaggaagcagtgaaacagcagcccagcagttctgagg
atggtcgtcctggaggcctccnagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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