XB-CLONE-3545560
Clone Name: IMAGE:7717169
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Gene:
slc13a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978628 [+]
aaacctagggcacctagatatttcacccaatactgatagagccattaaaatgtttacattatttttcttaaataaatacaagtgagatacttgccctgat
acaaaaaattaagttcttagagagaactttttacactcttgcctgctgggtattgcccttattaacagtactattaactgataaggtccccaattaaaag
ctttgcagagcagagtgagatgctacctcgactattctgtaggccggtagggtactcggcccatctgcttatagtcacagggtgggcacggcttgtgcgt
attcacggacactagagagtctgaactgtcaatctgtggattctggcagaagtcagtggggctgctgtaagatgccataaagaatcactgtttattgggc
tgatgggggactgtttgagtctcttggtagttgaaatgccagggtctattttgaattccagtctgaacctgtgccacatccaccactttatcatacttct
gaggctccttggcatccagttaaacacaactggccattaacagtccattacaaatgctaaggctgccctgtggagtttgcccaggatggaaatgtgccca
agtcgaacatgtaaaatccccagctgttaatggccagtgttatagtcagcacacccagaatgttgagcagtagtcctgcctttgccatgtcgataacctt
gagctgcccataagagaaggcaatggcattgggtggcgttgcaactggcagcataaaagccaagaggccgatagtgtgcatggaagcatgatatacagtg
gggtcagatggattgcttttggcatggatgctag
3' EST: CX978627 [+]ggaaatggatcttggcaaacaggaattatttcatcatctttttggtccctctctttcttctgcccctgcccctggtagtgcccaccaaggaggcaagctg
cggctttgtgatcattgtgatggcgttattctggtgcacagaagctcttcctctggccgtcactgcgctttttccagtcttgctttttccaatgatgggc
atcatggattccacagcggtgtgcagccagtatctgaaagacacgaacatgctgttcattggcgggctgctggttgcgatatcggtggagaaatggaatt
tgcataaacgcattgccctaagagtgctgctgattgttggagttaaacctgccctcctgcttcttggattcatggtggtcacagccttcctttccatgtg
gatcagtaacacagccacaacggccatgatgatacctattgcacaggctgtgctgcagcagcttcacacctcggaagagagagtggacaagccgtcagaa
gaaacaaaaaagaatgtgaatggaatggataatagcatgtatgaatcagtattgccaaatgggaaaatgatcctggcagtagaaaacacttatgctactg
taaacgagggatttgagatgcaagatagtactaaagatcctgagccttccaagctagaaaaagaaagtattggcccaatagtgatagaacttgaggatga
aaaacanaaggaagagaagcagaaacttagattggagaagcacctgaagatctgcaaaggaatgacccttagtgtctgttattctgccagcattggtgga
atgcaacattaactggcactaccccaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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